论文部分内容阅读
以葡萄‘左山一’(Vitis amurensis)与‘SO4’(V.berlandieri×V.riparia)杂交后代中耐碱性盐强的A15、A17与引进砧木中耐碱性盐能力较强的‘1103P’(V.rupestris×V.berlandieri)为试材,探讨耐碱性盐能力较强的葡萄砧木的荧光特性及光保护机制。将组培苗炼苗7周后每天浇灌100 mmol·L~(-1) NaHCO_3(pH 8.62),测定叶片电导率、丙二醛含量、叶绿素荧光、叶黄素循环及抗氰呼吸速率。NaHCO_3处理8 d后,各株系的叶片电导率与丙二醛(MDA)含量均有大幅升高;A15、A17的最大光化学效率(F_v/F_m)、光化学淬灭系数(q_P)的降低幅度均小于‘1103P’,且调节性能量耗散(Y_(NPQ))上升幅度较大,说明NaHCO_3胁迫下A15、A17的电子传递活性与光抑制程度受影响较小。在NaHCO_3处理下A15、A17和‘1103P’的叶黄素循环(A+Z)/(A+Z+V)分别比对照提高46.3%、37.0%、28.0%,A15、A17的抗氰呼吸速率在NaHCO_3处理下分别比对照提高141.5%、95.8%,‘1103P’则比对照提高63.0%。A15、A17通过依赖叶黄素循环的热耗散与增加抗氰呼吸速率来减轻光抑制以提高其耐碱性盐能力。
A15 and A17, which are resistant to alkaline salts in the progenies of crosses of Vitis amurensis and V. SO. (V. berlandieri × V.riparia), and ’1103P ’(V. rupestris × V.berlandieri) as the test material to explore the fluorescence characteristics and photoprotection mechanism of the grape rootstocks with high alkali tolerance ability. Seven days after the tissue culture seedlings were sprinkled, 100 mmol·L -1 NaHCO 3 (pH 8.62) was dripped daily. The leaf conductivity, malondialdehyde content, chlorophyll fluorescence, lutein cycle and cyanide respiration rate were measured. The leaf electrical conductivity and malondialdehyde (MDA) content increased sharply after treated with NaHCO_3 for 8 d. The maximum photochemical efficiency (F_v / F_m) and the decrease of q_P (q_P) of A15 and A17 Were all less than ’1103P’, and the increase of accommodative energy dissipation (Y_ (NPQ)) was larger, indicating that the electron transport activity and light inhibition of A15 and A17 were less affected by NaHCO 3. Under the NaHCO_3 treatment, the lutein cycle (A + Z) / (A + Z + V) of A15, A17 and ’1103P’ increased 46.3%, 37.0% and 28.0% Compared with the control, the content of NaHCO3 increased by 141.5% and 95.8%, respectively, and that of ’1103P’ increased by 63.0% compared with the control. A15, A17 reduce photoinhibition by increasing the resistance to cyanide respiration by relying on the thermal dissipation of the lutein cycle and increasing its resistance to alkaline salts.