论文部分内容阅读
通常,大鼠肺癌是长期地吸入中等或高水平的~(239)PuO_2所致。扫描电镜放射自显影技术提供了观察肺部α轨迹三维空间分布既快又廉价的方法。作者用30只年青、成熟的SPF雌性wister大鼠,给予鼻部一次性暴露在呈细雾状~(239)PuO_2粒子中。在吸入后14天通过~(169)Yb标记物全身测量最初肺泡~(239)PuO_2的沉积(IAD)。大鼠吸入钚粒子后,分组间隔一定时间处死动物,直到吸入后270天。经预处理后,在近心脏的肺叶处切取两张1mm厚的薄片,置4℃蒸馏水中24小时,干燥后,一张切片放在铝柱上,喷金-钯,扫描电镜观察。另一
In general, rat lung cancer is caused by long-term inhalation of moderate or high levels of ~ (239) PuO 2. Scanning electron microscopy autoradiography provides a fast and inexpensive way to observe the three-dimensional spatial distribution of the lung α trajectory. Thirty young and mature SPF female wister rats were given nasal exposures to ~ (239) PuO 2 particles in a fine mist. The initial alveolar 239 deposition of PuO 2 (IAD) was measured 14 days after inhalation via a ~ (169) Yb marker. After inhalation of plutonium particles, rats were sacrificed at intervals until 270 days after inhalation. After preconditioning, two 1mm thick slices were cut off near the heart lobes and placed in 4 ° C distilled water for 24 hours. After drying, a slice was placed on an aluminum column and sprayed with gold-palladium and scanning electron microscope. another