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[目的]探讨贝伐珠单抗对结直肠癌细胞血管内皮生长因子(VEGF)相关受体的影响。[方法]RT-PCR、Western blotting检测不同浓度贝伐珠单抗对结直肠癌肿瘤细胞VEGF相关受体表达水平的影响。[结果]与对照组相比,贝伐珠单抗药物组结直肠癌细胞的VEGF表达水平均明显下降(P<0.05);VEGFR1和VEGFR2表达水均明显上调(P<0.05)。[结论]贝伐珠单抗可引起了结直肠癌细胞VEGFR1和VEGFR2表达上调。
[Objective] To investigate the effect of bevacizumab on vascular endothelial growth factor (VEGF) -related receptors in colorectal cancer cells. [Methods] The effects of different concentrations of bevacizumab on the expression of VEGF-related receptor in colorectal cancer cells were detected by RT-PCR and Western blotting. [Results] Compared with the control group, the VEGF expression levels of colorectal cancer cells in bevacizumab group were significantly decreased (P <0.05), and the expression of VEGFR1 and VEGFR2 in water were significantly increased (P <0.05). [Conclusion] Bevacizumab can up-regulate the expression of VEGFR1 and VEGFR2 in colorectal cancer cells.