Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 ceils can inhibit tumor cell growth.Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes,the functions of DCX,and downstream genes in radiation therapy of neurogliocytoma.Stably transfected U87 cells were constructed (DCX-U87) and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions.Cells were irradiated using 60Co γ-ray at a dose rate of 1.0 Gy/min.Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed.Differentially expressed genes can correlate with radiation sensitivity and DCX transfection.DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy,but not the U87 cells,and their expression levels were higher in the 10 Gy group than in the 0 Gy group.The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.