论文部分内容阅读
探讨重组人骨形成蛋白-2(bone morphogenetic protein2,BMP-2)对肾缺血再灌注损伤(ischemia reperfusion injury,IRI)大鼠肾组织的影响。研究复制了大鼠肾IRI模型,并将Wistar大鼠随机分为假手术(S)组、肾IRI模型(R)组以及rhBMP-2预处理(于术前给予不同剂量rhBMP-2)组(B组),观察肾组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的含量,SOD活性、MDA含量,BUN、Scr含量以及肾功能和结构的变化。结果表明,与肾IRI模型组相比,rhBMP-2能显著降低肾组织中IL-6和IL-8的含量(P<0.05),rhBMP-2(4μg·kg-1)能显著降低肾组织中的TNF-α含量(P<0.05);亦能显著降低血浆中的BUN、Scr含量,增强肾组织中的SOD活性、降低肾组织中的MDA含量,使肾IRI大鼠的肾损害得到明显改善。与肾IRI模型组相比,rhBMP-2能显著减轻肾缺血再灌注损伤后肾组织结构的病变情况。由此可以推断,rhBMP-2可通过抑制肾组织促炎症细胞因子产生及抗氧化作用而减轻肾缺血再灌注损伤。
To investigate the effect of recombinant bone morphogenetic protein 2 (BMP-2) on renal tissue in rats with renal ischemia-reperfusion injury (IRI). The rat IRI model was duplicated and the Wistar rats were randomly divided into three groups: sham operation (S), IRI (R) and rhBMP-2 pretreatment (rhBMP-2 preoperatively) B group). The levels of TNF-α, IL-6 and IL-8, the activity of SOD and the content of MDA in BUN , Scr content and changes in renal function and structure. The results showed that rhBMP-2 significantly decreased the levels of IL-6 and IL-8 in kidney (P <0.05), and rhBMP-2 (4μg · kg-1) (P <0.05). It also significantly decreased the level of BUN and Scr in plasma, increased the activity of SOD in renal tissue and decreased the content of MDA in renal tissue, and markedly increased the renal damage in renal IRI rats improve. Compared with the renal IRI model group, rhBMP-2 can significantly reduce the renal tissue damage after renal ischemia-reperfusion injury. It can be inferred that rhBMP-2 can reduce renal ischemia-reperfusion injury by inhibiting proinflammatory cytokine production and anti-oxidative effects in renal tissues.