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通过花生籽仁不同时期转录组测序分析,从分子水平上揭示控制花生油脂合成的重要基因。本研究以高油和低油花生新品系为研究对象,构建了花生籽仁早期和中后期4个转录组测序文库。通过高通量测序共获得了59 236条Unigenes,其COG功能涉及了大多数的生命活动,整体功能类的基因最多有4 730条;其中与代谢类和油脂转运相关的基因有654条;Unigene参与的花生代谢通路可分为126类,其中涉及生化代谢的Unigene数量最多,达到了7 672条,占整体的32.95%;有4大类代谢与油脂相关,分别是脂肪酸的生物合成,涉及的基因有85条;脂肪酸的生化代谢,涉及的基因有145条;不饱和脂肪酸的生物合成途径,涉及116条基因;亚麻酸的代谢途径,涉及有138条基因。对花生籽仁两个不同发育时期的差异表达基因进行分析,共得到了120多种代谢途径(passway);并且籽仁发育中后期基因的表达量大部分下调,说明花生种子发育初期,各类调控脂肪酸合成的基因比较活跃,而随着合成油脂调控的不断继续,相关调节基因表达量下降,同时各类脂肪酸和油脂的合成也渐渐变慢。研究结果为深入揭示花生籽仁发育过程中油脂合成调控的相关基因及其功能提供了丰富的数据资源。
Through sequencing analysis of transcriptome in different stages of peanut kernel, the important genes controlling peanut oil synthesis were revealed at the molecular level. In this study, a new strain of high-oil and low-oil peanut was studied. Four transcriptome sequencing libraries of early and late peanut kernels were constructed. A total of 59 236 Unigenes were obtained by high-throughput sequencing. COG function involved most of the life activities, with up to 4 730 genes in the overall functional class, of which 654 were related to metabolism and lipid transport. Unigenes The involved peanut metabolic pathways can be divided into 126 categories, of which the largest number of Unigene involved in biochemical metabolism, reaching 7 672, accounting for 32.95% of the total; there are four major types of metabolism and fat related, namely fatty acid biosynthesis, involving There are 85 genes, fatty acid biochemical metabolism, involving 145 genes; unsaturated fatty acid biosynthesis pathway, involving 116 genes; linolenic acid metabolism, involving 138 genes. The differential expression genes of two peanut seed kernels at different developmental stages were analyzed. A total of 120 passways were obtained. The expression of genes in the mid and late stages of seed development was mostly down-regulated, indicating that during the early stage of peanut seed development, The genes regulating fatty acid synthesis are relatively active. However, with the continuous regulation of synthetic fat, the expression of related regulatory genes is decreased, and the synthesis of various fatty acids and oils gradually slow down. The results provide abundant data resources for further revealing the related genes and functions of oil synthesis and regulation during the development of peanut kernel.