以河北杨体细胞抗盐性变异系再生植株试管苗为材料，采用聚丙烯酸胺凝胶电泳方法，对７个变异系及其对照系的过氧化物酶同工酶、酯酶同工酶、苹果酸脱氢酶同工酶、谷氨酸脱氢酶同工酶、异柠檬酸脱氢酶同工酶、乳酸脱氨酶同工酶进行了分析。结果表明，每个变异系与对照系至少在一个酶谱上存在变异，从而证明７个变异系均系突变体。 In this study, the in vitro plantlets regenerated from salt-tolerant mutant of Hebei poplar were cultured in vitro using polyacrylamide gel electrophoresis. The activities of peroxidase isoenzyme, esterase isoenzyme, Malate dehydrogenase isozyme, glutamate dehydrogenase isozyme, isocitrate dehydrogenase isozyme and lactate deaminase isozyme were analyzed. The results showed that there was at least one variation in each zymogram for each mutant line and the control line, which proved that seven mutant lines were homogenous mutants.