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目的 :研究N 亚硝基 肌氨酸乙酯 (N nitrososarcosineethylester,NSEE)诱导NIH小鼠前胃癌变过程中 ,热休克蛋白 70 (heatshockprotein 70 ,Hsp70 )和增殖细胞核抗原 (proliferatingcellnu clearantigen ,PCNA)的表达及意义。方法 :将 14 4只小鼠分为6组 ,通过用NSEE灌胃建立小鼠前胃癌模型。每隔2wk处死 2 4只小鼠 ,取其前胃用免疫组化ABC法 ,对小鼠前胃癌变进程中细胞形态的改变 ,Hsp70和PCNA的表达进行跟踪研究。结果 :在NSEE诱癌过程中 ,Hsp70的表达呈现马鞍型 ,而Hsp70的表达一直呈增强的趋势。给药后 5 6、70d ,与对照组比较差异显著 (P <0 .0 5 ) ,84d差异极显著(P <0 .0 1)。PCNA的表达随着诱癌进程呈现递增趋势 ,给药后 4 2、5 6d ,与对照组比较差异显著 (P <0 .0 5 ) ,70、84d差异极显著 (P <0 .0 1)。Hsp70与PCNA的表达呈正相关关系 (r=0 .98,P <0 .0 1)。结论 :在诱发NIH小鼠前胃癌变过程中 ,Hsp70、PCNA的表达均呈现升高趋势 ,且两者呈正相关关系
OBJECTIVE: To study the expression of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the process of gastric carcinogenesis induced by N-nitrososarcosineethylester (NSEE) in NIH mice. And meaning. Methods: One hundred and forty-four mice were divided into six groups. The mouse gastric cancer model was established by intragastric administration of NSEE. Twenty-four mice were sacrificed at 2wk intervals, and their gastric anastomosis was used to observe the changes of cell morphology, the expression of Hsp70 and PCNA in mouse gastric precancerous lesions. Results: During the induction of NSEE, the expression of Hsp70 presented a saddle type, while the expression of Hsp70 showed an increasing trend. There was significant difference between the control group and the control group (P <0.05) on the 56th and 70th day after administration, and the difference was significant on the 84th day (P <0.01). The expression of PCNA showed an increasing trend with the progress of cancer induction. The difference was significant at 70 and 84 days after administration (P <0.01) at day 4 and day 6 after dosing compared with the control group (P <0. 05) . Hsp70 and PCNA expression was positively correlated (r = 0.98, P <0.01). CONCLUSION: The expression of Hsp70 and PCNA in NIH mice during the process of gastric precancerous lesions showed a rising trend, and the two were positively correlated