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美国Webley等为了解决儿茶酚胺是否与灵长类卵巢孕酮生成有关,进行了以下实验。他们从进行体外授精病人的卵泡中抽吸颗粒细胞,在补充血清的培养介质中进行离体培养3～4天,结果发现,肾上腺素和去甲肾上腺素可促进颗粒细胞分泌孕酮,其程度与所用剂量(10~(-7)～10~(-4)mol/L)相关,在浓度为10~(-5)mol/L时,促进作用最强,而且此反应可被β-拮抗剂心得安(10~(-5)mol/L)所阻止。肾上腺素(10~(-5)mol/L)和人类绒毛膜促性腺激素(10和100ng/ml,培养6天)促进孕酮分泌的特点相似,但两者无协同作用。褪黑激素(200kg/ml)也促进孕酮分泌,与肾上腺素相似,此促进作用也可被心得安(10~(-(?))mol/L)所阻止。肾上腺素和褪黑
The United States Webley, etc. In order to find out whether catecholamines and prion progesterone ovarian formation, the following experiment. They inhaled granulosa cells from the follicles of patients who were fertilized in vitro and cultured ex vivo in culture medium supplemented with serum for 3-4 days and found that epinephrine and norepinephrine promoted the secretion of progesterone by granulosa cells (10 ~ (-7) ~ 10 ~ (-4) mol / L), the strongest effect was found at the concentration of 10 ~ (-5) mol / L, and this reaction could be blocked by β-antagonist Agent propranolol (10 ~ (-5) mol / L) blocked. Adrenaline (10 -5 mol / L) and human chorionic gonadotropin (10 and 100 ng / ml, cultured for 6 days) promoted progesterone secretion with similar characteristics, but no synergistic effect. Melatonin (200 kg / ml) also promotes progesterone secretion, which is similar to epinephrine, and is prevented by propranolol (10 ~ (-)?) Mol / L). Adrenaline and melatonin