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北京医科大学附属第三医院心血管研究室韩启德在美国访问和工作期间,与美国学者合作,采用受体结合实验与生理、药理和生化等功能实验相结合的方法,从多种途径对α_1肾上腺素受体亚型问题进行了深入的研究,发现:(1)在受体结合实验中,将细胞膜标本与10μmol/L chlorethyl clonidine(CEC,一种不可逆性α_1受体拮抗剂)预孵育10min,使与~(125)I-BE 2254(IBE)特异性结合的位点在大鼠肝和脾几乎全部丧失,而在海马回、输精管、尾动脉、肾、大脑皮层和心脏则只丧失41～65%。在大鼠离体脾和输精管的功能性实验中,将组织与100μmol/L CEC预孵育30min,使去甲肾上腺素(NE)引起脾收缩的敏感性和
Han Qide, Cardiovascular Research Laboratory, Third Affiliated Hospital of Beijing Medical University During his visit and work in the United States, Han Qi-de cooperated with American scholar and used a combination of receptor-binding experiments with functional experiments such as physiology, pharmacology and biochemistry. (1) In the receptor binding experiments, the cell membrane specimens were pre-incubated with 10 μmol / L chlorethyl clonidine (CEC, an irreversible α 1 receptor antagonist) for 10 min, The site of specific binding to 125E-BE 2254 (IBE) was almost completely lost in the liver and spleen of rats, whereas in the hippocampus, the vas deferens, tail artery, kidney, cerebral cortex and heart, only 41 ~ 65%. In the functional experiment of rat spleen and vas deferens in vitro, the tissue was pre-incubated with 100 μmol / L CEC for 30 min to sensitize noradrenalin (NE)