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1990年配制广杂和品种02428、轮回422与籼粳恢复系杂种F1，翌年对广亲和品种与籼粳恢复系杂种F1进行花药培养获得纯会二倍体花粉再生植株．以粳不育系寒丰A，籼不育系珍汕97A、籼粳测验种南京11、南特、Balilla、秋光为母本与花粉再生植株测交，鉴定筛选广亲和恢复系．结果表明在籼恢和广亲和品种杂交F1的花培后代中，对寒丰A和珍汕97A具有强恢复力的花粉再生植株分别占36．67％和64．67％．衍生于粳恢和广亲和品种杂种动的花粉植株中，分别有71．26％和32.34％的植株对寒丰A和珍汕97A具有强恢复力．从317份粳恢或籼恢和广杂和品种杂种F1花粉再生植株中筛选出15个农艺性状优良的广杂和恢复系．部分衍生于花药培养的广亲和恢复系与寒丰A、珍汕97A测交，杂种F1农艺和产量性状具有明显的杂种优势，其中广亲和恢复系H17、H158和H281表现出优良的广亲和性和广谱恢复性。遗传分析表明这3个广亲和恢复系的广亲和性由一对显性生效基因控制，符合孢子体-配子体互作模式．花药培养是迅速改良和培育水稻广亲和恢复系的有效途径之一．
In 1990, the hybrid variety 02428, Rei 422 and the indica-japonica restorer F1 were prepared, and the diploid pollen regenerated plants were obtained by anther culture of F1 hybrid between F1 and F1. Han-A line of japonica CMS, Zhenshan 97A of indica CMS line, Nanjing 11, Nante, Balilla and Qiu-Guang as female parent and pollen regenerated plants were identified and identified. The results showed that pollen regenerated plants with strong resilience to cold Feng A and Zhenshan 97A accounted for 36.67% and 64.67%, respectively, in the F1 progenies of F1 hybrids. Among the pollen plants derived from japonica hybrids and broad-compatibility hybrids, 71.26% and 32.34% of the plants had strong resilience to both Hanfeng A and Zhenshan 97A, respectively. A total of 15 agronomic traits and restorer lines were screened from 317 Japonica restorer or indica hybrids and F1 hybrids. Some broad-compatibility restorer lines derived from anther culture were identified as hybrids with Hanfeng A and Zhenshan 97A. The agronomic and yield traits of hybrid F1 had obvious heterosis. Among them, the broad-compatibility restorer lines H17, H158 and H281 showed excellent broadness Affinity and broad spectrum recovery. Genetic analysis showed that the wide compatibility of these three broad-compatibility restorers was controlled by a pair of dominant-effect genes, which conformed to the sporophyte-gametophyte interaction pattern. Anther culture is one of the effective ways to rapidly improve and cultivate a wide compatibility restorer line in rice.