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目的:探讨大剂量甲基强的松龙(MPS)治疗大鼠急性脊髓损伤(ASCI)后,胶质纤维酸性蛋白(GFAP)的变化.方法:44只成年雄性WISTAR大鼠,随机分成对照、损伤和治疗3组.采用ALEN打击法损伤脊髓.损伤组与治疗组于损伤后15MIN内尾静脉内推注MPS30MG/KG,以后23H内静滴MPS5.4MG/KG?H－1.各组动物分别于损伤后1、4、8D处死,行GFAP单克隆抗体免疫细胞化学染色.结果:对照组大鼠应用MPS后4D,GFAP减少到80％,8D后减少到63％.ASCI后,各组GFAP增加,治疗组与损伤组GFAP之比损伤后第1D为87.2％,第4D为88.6％,第8D为95.7％.结论:大剂量MPS抑制未损伤大鼠GFAP的合成.对ASCI后,GFAP含量的增加也有一定的抑制作用.“,”Objective: Our aim was to study the effect of highdose of methylprednisolone on glial fibrillary acidic protein after acute spinal cord injury in rats. Methods: Immunocytochemistry and image analysis methods were used to detect the change of the glial fibrillary acidic protein (GFAP) in astrocyte in acute spinal cord injury rats which were early treated by intravenous highdose of methylprednisolone. 44 male Wistar rats were randomly assigned to control,injury and ...