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目的:研究连翘酯苷对顺铂作用后豚鼠耳蜗c-jun表达的影响。方法:将30只豚鼠随机分为对照组(10只),顺铂组(10只)和连翘酯苷组(10只)。腹腔注射顺铂溶液(8mg/kg),1次/d,连续7d,建立顺铂耳毒性模型;连翘酯苷组在每次注射顺铂溶液30min前腹腔注射连翘酯苷25.0mg/kg/d,连续7d;对照组以生理盐水代替顺铂溶液注射,连续7d。实验动物被处死前,检测其DPOAE幅值变化;采用蛋白质印迹杂交(Western Blotting)检测各组豚鼠耳蜗c-jun蛋白的表达,逆转录聚合酶链反应检测各组豚鼠耳蜗c-jun基因mRNA的表达。结果:顺铂组DPOAE幅值明显低于对照组(P<0.01);相比于顺铂组,连翘酯苷组DPOAE幅值明显升高(P<0.05)。顺铂组豚鼠耳蜗c-jun蛋白与mRNA表达水平均显著高于对照组(P<0.01);相比于顺铂组,连翘酯苷组c-jun蛋白与mRNA表达水平明显降低(P<0.05)。结论:连翘酯苷能够通过降低c-jun的表达防护顺铂所致的耳蜗损伤。
Objective: To study the effect of forsythiaside on c-jun expression in guinea pig cochlea after cisplatin treatment. Methods: Thirty guinea pigs were randomly divided into control group (n = 10), cisplatin group (n = 10) and forsythiaside group (n = 10). Cisplatin ototoxicity model was established by intraperitoneal injection of cisplatin solution (8mg / kg) once a day for 7 days. Forsythiaside group was injected intraperitoneally with 25.0mg / kg of Forsythiaside / d for 7 days. The control group was injected with cisplatin instead of cisplatin for 7 days. The DPOAE amplitude of guinea pig cochlea was detected by Western Blotting and c-jun mRNA expression in cochlea of guinea pigs were detected by reverse transcription-polymerase chain reaction expression. Results: The amplitude of DPOAE in cisplatin group was significantly lower than that in control group (P <0.01). Compared with cisplatin group, the amplitude of DPOAE in Forsythiaside group was significantly increased (P <0.05). The expression of c-jun protein and mRNA in Cisplatin group was significantly higher than that in control group (P <0.01). Compared with Cisplatin group, the expression of c-jun protein and mRNA in Forsythiaside group was significantly decreased (P < 0.05). Conclusion: Forsythiaside can protect Cochlear-induced cochlear injury by decreasing the expression of c-jun.