论文部分内容阅读
目的 探讨α 病毒RNA复制酶对基因疫苗的免疫学效应的加强作用 ,寻找更好的基因疫苗形式。方法 将小鼠肥大细胞瘤P815的肿瘤特异抗原P1A基因克隆到含SFV的RNA复制酶的真核表达载体pSMART2a中 ,以此作为肿瘤基因疫苗 ,观察该疫苗对P815种植瘤的防治作用、特异细胞毒T淋巴细胞激活和抗体的生成情况。结果 该重组基因疫苗在体外有很好的表达 ,注射后CTL的最大杀伤效率为 60 % ,而普通载体疫苗形式pCI neo P1A则只有 40 %的杀伤活性 ;在观察期限内 ,前者的动物成瘤率和动物生存率分别为 2 0 %和 40 % ,后者则分别为 60 %和 2 0 %。两种情况下都不能检测到任何特异抗体的产生。结论 α 病毒RNA复制酶对基因疫苗的免疫学效应有加强作用。
Objective To investigate the role of α-viral RNA replicase in enhancing the immunological effects of gene vaccines and to find out better forms of gene vaccines. Methods P1A gene of mouse mastocytoma P815 was cloned into eukaryotic expression vector pSMART2a containing SFV RNA replicase and used as a tumor gene vaccine to observe the preventive and therapeutic effects of the vaccine on P815 implants. T-lymphocyte activation and antibody production. Results The recombinant DNA vaccine had good expression in vitro. The maximum cytotoxicity of CTL after injection was 60%, but only 40% of pCI neo P1A was found in common vector vaccine. During the observation period, Rates and animal survival rates were 20% and 40% respectively, while the latter were 60% and 20% respectively. No specific antibody production could be detected in either case. Conclusion The alphavirus RNA replicase can enhance the immunological effect of gene vaccines.