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用离子交换层析法一步纯化重组人白细胞介素４，该方法简单易行，对样品不产生稀释作用；所得产品具有高纯度（≥９５％），高生物学活性（≥１０ＭＵ／ｍｇ）和低毒性（内毒素含量≤０．５ＺＵ／Ｌ）的特点，可满足实验室工作的需要．当与凝胶过滤法结合使用时，所得产品纯度可达９８％，生物学活性进一步提高．
The recombinant human interleukin-4 was purified by ion-exchange chromatography in one step. The method is simple and easy to perform, and has no dilution effect on the sample. The obtained product has high purity (≥95%), high biological activity (≥10MU / mg) Low toxicity (endotoxin content ≤ 0.5ZU / L) features to meet the needs of laboratory work. When used in combination with gel filtration, the purity of the product obtained is up to 98% and the biological activity is further increased.