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ＩＬ－１２是一种具有广泛免疫调节作用和抗肿瘤作用的细胞因子，研究了ＩＬ－１２对白介素４基因转染的小鼠Ｂ１６黑色素瘤细胞（Ｂｌ６－ＩＬ－４￣＋）诱导的ＣＴＬ的杀伤活性、ＩＦＮ－γ分泌水平和增殖能力的影响。结果发现，ＩＬ－１２能显著增强体外诱导的ＣＴＬ的杀伤活性及提高ＩＦＮ－γ的分泌水平，并能促进ＣＴＬ的增殖。ＩＬ－１２与低剂量的ＩＬ－２（５０Ｕ／ｍｌ）合用能协同增强ＣＴＬ的杀伤活性、ＩＦＮ－γ分泌水平及增殖能力， 但与高剂量的ＩＬ－２（５００Ｕ／ｍｌ）合用却降低了促ＣＴＬ诱导作用，结果表明，ＩＬ－１２ 作为瘤苗增强剂可望促进肿瘤细胞靶向的ＩＬ－４基因治疗的效果，与低剂量的ＩＬ－２合用后效果更佳。
IL-12 is a cytokine that has a wide range of immunoregulatory and antitumor effects. IL-12 induced CTL induced by interleukin 4 gene transfected mouse B16 melanoma cells (Bl6-IL-4~+) The effect of killing activity, IFN-γ secretion level and proliferation ability. As a result, it was found that IL-12 can significantly enhance the killing activity of CTL induced in vitro and increase the secretion level of IFN-γ, and can promote the proliferation of CTL. IL-12 combined with low doses of IL-2 (50 U/ml) synergistically enhanced CTL killing activity, IFN-γ secretion, and proliferation but decreased with high doses of IL-2 (500 U/ml). The induction of CTL induction, the results show that IL-12 as a tumor enhancer is expected to promote tumor cell-targeted IL-4 gene therapy, combined with low-dose IL-2 better.