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以转TaEBP基因小麦新品系G258及其受体亲本衡观35种子为材料,研究了20%PEG人工模拟干旱胁迫对参试材料种子萌发期的胚芽鞘长度、胚根数、主胚根长度的影响。在此基础上,结合盆栽控水法研究了水分胁迫对转基因小麦新品系生长发育中后期抗旱相关生理生化及形态指标的影响。结果显示:在20%PEG模拟干旱胁迫下,参试材料的胚芽鞘长、胚根数、主胚根都有所降低,而G258降幅较小,并且在同一胁迫下胚芽鞘长度、胚根数、主胚根指标都优于HG35;盆栽控水条件下,随着水分胁迫加剧和生长发育的推进,参试材料的水分利用率呈下降趋势,而转基因小麦G258下降幅度较小。丙二醛含量呈上升趋势,对照品种HG35上升幅度更大,G258上升幅度较小;在中度和重度胁迫下,转基因小麦G258每一生育期脯氨酸、ABA含量都明显高于对照品种HG35。上述结果表明,转基因小麦新品系G258具有较强的抗旱节水性能。
The effects of 20% PEG simulated drought stress on the coleoptile length, radicle number, main radicle length of germplasm in the germination stage of the seed germplasm were studied by using transgenic G258 with TaEBP gene and its receptor parents, influences. Based on this, the effects of water stress on the physiological and biochemical parameters related to drought resistance at the late growth stage of transgenic wheat were studied by combining with potted water control. The results showed that under the 20% PEG simulated drought stress, the length of the cole sheath, the number of radicles and the main radicle of the tested materials decreased, but the decrease of G258 was smaller. Under the same stress, the length of coleoptile, , And the main radicle indexes were better than HG35. Under the conditions of potted water control, with the increase of water stress and the promotion of growth and development, the water use efficiency of the tested materials showed a decreasing trend, but the decrease of G258 of transgenic wheat was less. The content of malondialdehyde (MDA) increased with the increase of HG35 and the increase of G258. Under moderate and severe stress, the contents of proline and ABA in transgenic wheat G258 were significantly higher than that of HG35 . The above results show that the new transgenic wheat line G258 has strong drought-resistant and water-saving properties.