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以传统的检测方法为对照经过一系列的筛选后 ,在国内首次将病毒的dsRNA检测分析引入果树无病毒苗的检测方法之中 ,确立了葡萄病毒病dsRNA的提取步骤 ,包括试剂选择、浓度的设定、操作方法的确立 ,与国外文献相比有多方面的改进 ,使其更加简单、实用 ,并在实际的应用当中取得成功。在确立方法的基础上对生产中广泛发生的葡萄卷叶病病毒 (GLRv)进行检测并确立检测标准 ,为今后葡萄病毒病的诊断检测奠定了良好的基础
After a series of screening tests using the traditional detection methods, the dsRNA detection and analysis of the virus was first introduced into the detection method of virus-free seedlings of fruit trees in China for the first time, and the steps of extracting dsRNA from grape virus diseases were established, including reagent selection, concentration The establishment and operation of the method have many improvements compared with the foreign literature, making it more simple and practical and successful in practical application. Based on the establishment of the method, the grapevine leaf curl virus (GLRv), which is widely produced in the production, is detected and the test standard is established, which lays a good foundation for the diagnosis and detection of the grapevirus disease in the future