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目的研究大果木姜子镇痛作用的成分和机制。方法昆明种小鼠随机分为正常对照组、安慰剂组、阿司匹林组、曲马多组和大果木姜子石油醚、二氯甲烷、正丁醇提取部位的高、中、低剂量组。除正常组外,各组灌胃给药,安慰剂组灌服生理盐水。①扭体法:给药后45min腹腔注射0.6%醋酸0.2mL/g,观察记录给药后15min内小鼠扭体次数。②热板法:雌性小鼠,灌胃给药2h后,以热板法测定小鼠的痛觉反应时间。③甲醛法:给药2h后,小鼠左后足底注射20μL2.5%福尔马林,测定小鼠5～10min、20～30min的痛觉反应。结果①大果木姜子各提取部位对醋酸诱发小鼠扭体反应有显著的镇痛作用,与安慰剂组比较,石油醚提取部位对小鼠的扭体次数减少明显(P<0.01)。②大果木姜子石油醚提取部位、二氯甲烷提取部位对热板法致痛有显著的镇痛作用,与安慰剂组比较,能显著提高小鼠的痛阈值(P<0.01)。③大果木姜子石油醚提取部位对甲醛致痛有显著的镇痛作用,能明显减少小鼠5～10min(I相)和20～30min(II相)的痛觉反应时间,与安慰剂组比较,镇痛效果有显著性差异(P<0.05或P<0.01)。结论大果木姜子脂溶性成分具有镇痛作用,其镇痛作用机制可能与中枢机制有关。
Objective To study the components and mechanism of the analgesic effect of Zingiber officinale. Methods Kunming mice were randomly divided into high, medium and low dose groups of normal control group, placebo group, aspirin group, tramadol group and large fruiting tree ginger petroleum ether, methylene chloride and n-butanol extracts. In addition to the normal group, each group intragastric administration, placebo group fed with saline. ① writhing: 45min after administration of intraperitoneal injection of 0.6% acetic acid 0.2mL / g, observed and recorded within 15min after administration of the number of writhing in mice. ② hot plate method: female mice, gavage administered 2h, the hot plate method to determine the pain response time of mice. ③ Formaldehyde method: After 2h administration, 20μL 2.5% formalin was injected into the left posterior plantar of mice, and the pain response of mice was determined at 5 ~ 10min and 20 ~ 30min. Results (1) The extracts from Zingiber officinale showed a significant analgesic effect on the writhing reaction induced by acetic acid in mice. Compared with the placebo group, the number of writhing was significantly decreased (P <0.01). (2) Fructus arboricus extracted with petroleum ether and methylene chloride had a significant analgesic effect on the hot plate method. Compared with the placebo group, the pain threshold of mice increased significantly (P <0.01). (3) The extraction of petroleum jelly from petroleum jelly of Aristolochia grandis has a significant analgesic effect on the pain induced by formaldehyde, and can significantly reduce the pain response time of mice in 5 to 10 minutes (I phase) and 20 to 30 minutes (II phase), compared with placebo group , Analgesic effect was significantly different (P <0.05 or P <0.01). CONCLUSION: The fat-soluble components of Zingiber officinale have the analgesic effect and the mechanism of analgesic action may be related to the central mechanism.