论文部分内容阅读
从江苏省句容市丘陵山区未施用过多菌灵（ＭＢＣ）的白菜型油菜菌核病株上，采集了４９个Ｓｃｌｅｒｏｔｉｎｉａｓｃｌｅ－ｒｏｔｉｏｒｕｍ野生菌株。经测定，ＭＢＣ抑制这些菌株菌丝生长的平均ＥＣ５０为０．２１９８±０．１０８３μｇ／ｍｌ，ＭＩＣ值为５．０μｇ／ｍｌ。从镇江农科所油菜育种组试验田中采集的Ｓ．ｓｃｌｅｒｏｔｉｏｒｕｍ菌核标样中，发现６个抗ＭＢＣ的抗性菌株，这６个菌株的ＥＣ５０值均大于２０００μｇ／ｍｌ。研究表明：这６个抗性菌株经无性世代、有性世代，或在低温（４℃）中贮存１０个月后，其抗药性都没有丧失或降低；抗性菌株对离体油菜叶片的致病力与野生的敏感菌株没有差异；油菜叶片经１０００μｇ／ｍｌＭＢＣ溶液浸渍处理后，敏感菌株对其都不能侵染，而抗性菌株对这些叶片的致病力与对未浸药叶片的致病力无明显差异
A total of 49 wild strains of Sclerotiniascle-rotiorum were collected from the B. rapae sclerotinia without applying carbendazim (MBC) in the hilly region of Jurong City, Jiangsu Province. The mean EC50 of MBC inhibiting the mycelial growth of these strains was determined to be 0.2198 ± 0.1083 μg / ml and the MIC value was 5.0 μg / ml. S from Zhenjiang Institute of Science rapeseed breeding field experimental field collected S. Six sclerotiorum sclerotiorin-resistant samples were found to have six anti-MBC resistant strains with EC50 values greater than 2000 μg / ml. The results showed that all six resistant strains had no loss of resistance or decreased their resistance after being stored for 10 months in sexual generation, sexual generation, or in low temperature (4 ℃). The resistance of the resistant strains to in vitro rape leaves There was no difference between the susceptible and wild sensitive strains. After being inoculated with 1000μg / ml MBC solution, the susceptible strains could not infect them. However, the pathogenicity of the resistant strains to these leaves was not related to the pathogenicity No significant difference in force