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如何准确预测GVHD的发生始终是造血干细胞移植领域中十分棘手的难题。采用HLA抗原三维结构的模型来预测GVHD的发生是移植免疫研究的最新进展之一。我们在对17个移植家系进行筛选时,发现了一个供、受体HLA-A位点不合的家系,临床移植后该患者发生了Ⅳ级GVHD。本实验采用高分辨巢式PCR-SSP和DNA序列测定证实患者的HLA-A位点发生了重组(A~*6901→A~*0201)。进行蛋白质三维结构模型分析显示,供、受体差异抗原的空间构象存在明显差异。可能会成为移植后发生GVHD的分子基础,从而验证了我们的实验结果和理论推断。由此可见,该HLA抗原三维结构模型在预测GVHD的发生中具有重要意义。
How to accurately predict the occurrence of GVHD has always been a very difficult problem in the field of hematopoietic stem cell transplantation. Predicting the occurrence of GVHD using the model of three-dimensional structure of HLA antigen is one of the latest advances in the study of transplantation immunity. When we screened 17 transplant families, we found a pedigree with donor-recipient HLA-A loci that developed grade IV GVHD after clinical transplantation. In this experiment, high-resolution nested PCR-SSP and DNA sequencing confirmed the patient’s HLA-A site was recombined (A * 6901 → A ~ * 0201). Three-dimensional protein structural model analysis showed that there was a significant difference in the spatial conformation of donor and recipient differentially expressed antigens. May become the molecular basis of GVHD after transplantation, thus verifying our experimental results and theoretical inference. Thus, the three-dimensional structural model of HLA antigen in the prediction of the occurrence of GVHD is of great significance.