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Objective: To investigate the distribution of substance P receptor (SPR) in the brainstem auditorycentre and medial geniculate body (MGB) of guinea pigs. Methods: Rabbit antiserum against SPR, immunohistochemical method and glucose-oxidase-DAB-nickle technique were used in this study. Results:SPR-positiveneurons, fibers and terminals were distributed widely in the cochlear nucleus, superior olive complex,inferiorcolliculus and MGB. In the cochlear nucleus, SPR-positive neurons were distributed mainly in the ventralcochlear nucleus (VCN). SPR-positive products were located predominately on the cell membrane. The density of positive terminals in the dorsal cochlear nucleus (DCN) was higher than that in the ventral cochlear nucleus. The distribution pattern of SPR-positive products in the superior olive complex had distinct characteristics. SPR-positive fibers, terminals and neurons were also found, but the number of positive neurons wasfewer. The density of SPR-positive fibers and terminals in the MGB was quite high. Conclusion:SP may participate in transmitting sound messages in brainstem auditory centre and MGB of the guinea pig.
Methods: To investigate the distribution of substance P receptor (SPR) in the brainstem auditorycentre and medial geniculate body (MGB) of guinea pigs. Methods: Rabbit antiserum against SPR, immunohistochemical method and glucose-oxidase-DAB-nickle technique were used in this study. Results: SPR-positive neurons, fibers and terminals were distributed widely in the cochlear nucleus, superior olive complex, inferiorcolliculus and MGB. In the cochlear nucleus, SPR-positive neurons were distributed mainly in the ventral cochlear nucleus (VCN) products were located predominately on the cell membrane. The density of positive terminals in the dorsal cochlear nucleus (DCN) was higher than that in the ventral cochlear nucleus. The distribution pattern of SPR-positive products in the superior olive complex had distinct characteristics. -positive fibers, terminals and neurons were also found, but the number of positive neurons wasfewer. The density of SPR-positive fibers and termin als in the MGB was quite high. Conclusion: SP may participate in transmitting sound messages in brainstem auditory center and MGB of the guinea pig.