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本实验以Ｌ－赖氨酸产生菌钝齿棒杆菌（Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍｃｒｅｎａｔｕｍ）ＯＭ－４（ＨＳ＋，ＡＥＳｒ，Ｍｅｔｒ）为出发菌株，经硫酸二乙酯和氯化锂复合诱变处理，用ＡＥＣ（Ｓ－［２－氨乙基）－Ｌ－半胱氨酸）和苏氨酸定向筛选获得了高抗ＡＥＣ的突变株ＡＴ－５（ＨＳ＋，ＡＥＣｒ，Ｍｅｔｒ）菌株，再以ＡＴ－５为出发菌株经亚硝基胍诱变处理，获得一株产Ｌ－赖氨酸的ＴＡ（２－噻唑－ＤＬ－丙氨酸）抗性突变株（ＡＴＴ一６８号），菌株产酸量由原来ＯＭ一４菌株的４６．３ｍｇ／ｍＬ提高到７４．８ｍｇ／ｍＬ提高１６１．５％，在筛选结构类似物抗性突变株时采用小钢杯法，可大大节省结构类似物药品．
In this experiment, L-lysine-producing bacteria Corynebacterium crenatum OM-4 (HS +, AESr, Metr) was used as a starting strain, treated with diethyl sulfate and lithium chloride, mutagenized with AEC (S- (HS +, AECr, Metr) strains with high anti-AEC activity were obtained by targeted screening of [2-aminoethyl) -L-cysteine and threonine. AT- Nitrosoguanidine mutagenesis to obtain a L-lysine-producing TA (2-thiazole-DL-alanine) resistance mutant (ATT-68), the strain acid production from the original OM a 4 The 46.3mg / mL of the strain increased to 74.8mg / mL, which increased by 161.5%. The small steel cup method was used to screen structural analogue resistant mutant strains, which could greatly save structural analogs.