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目的 探讨重组白喉毒素-人白细胞介素13嵌合毒素DT389-IL13对胶质瘤细胞的杀伤作用机制。方法 在U251细胞中加入不同浓度的嵌合毒素DT389-IL13,作用72 h后分别进行H33342染色,观察胶质瘤细胞核形态,电镜观察细胞超微结构,用流式细胞仪计数亚二倍体细胞百分数等。结果 3种检测结果均表明,嵌合毒素DT389-IL13可诱导U251胶质瘤细胞发生凋亡,1×10-9mol/L嵌合毒素作用72 h后亚二倍体细胞百分比为38％,1×10-8mol/L.作用后亚二倍体细胞百分比为99％,明显高于对照组(3.63％),与荧光染色观察的结果一致;电镜下还可见固缩的线粒体。结论 嵌合毒素DT389-IL13可诱导胶质瘤细胞凋亡。
Objective To investigate the mechanism of killing effect of recombinant diphtheria toxin-human interleukin 13 chimeric toxin DT389-IL13 on glioma cells. Methods Different concentrations of chimeric toxin DT389-IL13 were added to U251 cells. H33342 cells were stained for 72 hours. The morphology of glioma cells was observed. The ultrastructure of glioma cells was observed by electron microscopy. The sub-diploid cells were counted by flow cytometry Percentage and so on. Results The results of the three assays showed that the chimeric toxin DT389-IL13 could induce the apoptosis of U251 glioma cells, the percentage of sub-diploid cells was 38% after 1 × 10-9 mol / L chimeric toxin for 72 h × 10-8mol / L. After treatment, the percentage of sub-diploid cells was 99%, which was significantly higher than that of the control group (3.63%), which was consistent with the results of fluorescence staining. Conclusion Chimeric toxin DT389-IL13 can induce glioma cell apoptosis.