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目的研究磷丙泊酚钠后处理对大鼠肝脏缺血再灌注损伤的保护作用及可能机制。方法 48只Sprague-Dawley大鼠,随机分4组,即假手术组(S组)、对照组(C组)、丙泊酚组(P组)、磷丙泊酚钠组(F组)。每组再根据再灌注2 h或4 h分为2个亚组,分别为S2h、C2h、P2h、F2h亚组和S4h、C4h、P4h、F4h亚组,每个亚组6只大鼠。肝脏缺血1 h后恢复再灌注,S组和C组再灌注开始给予生理盐水静脉泵注,P组给予丙泊酚静脉泵注,F组给予磷丙泊酚钠静脉泵注。于肝脏缺血1 h和再灌注结束后,取血测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST),同时取左肝石蜡包埋切片测定B淋巴细胞瘤/白血病-2(bcl-2)蛋白、bcl-2相关X蛋白(bax)含量,苏木精-伊红染色后观察形态学,计数坏死肝细胞比例评价损伤程度。结果 C2h、P2h、F2h和C4h、P4h、F4h亚组血清ALT、AST值,坏死细胞比例和肝组织bcl-2和bax蛋白含量分别高于S2h、S4h亚组(P<0.05),P2h、F2h和P4h、F4h亚组ALT、AST值、坏死细胞比例和肝组织bax蛋白含量低于C2h、C4h亚组(P<0.05),bcl-2蛋白含量分别高于C2h、C4h亚组(P<0.05)。结论磷丙泊酚钠对肝脏缺血再灌注损伤有保护作用,其保护机制可能和调控bcl-2、bax蛋白的表达有关。
Objective To study the protective effect of sodium and phosphorus propofol sodium postconditioning on hepatic ischemia-reperfusion injury in rats and its possible mechanism. Methods Forty eight Sprague-Dawley rats were randomly divided into four groups: sham operation group (S group), control group (C group), propofol group (P group), and propofol sodium group (F group). Each group was divided into two subgroups: S2h, C2h, P2h and F2h subgroups and S4h, C4h, P4h and F4h subgroups, 6 rats in each subgroup according to 2 h or 4 h reperfusion. Rats in ischemia group were reperfused 1 h after ischemia. Rats in groups S and C were intravenously infused with saline by intravenous injection. Propofol was infused intravenously in group P, and propofol sodium was intravenously injected in group F, respectively. Blood samples were collected for determination of ALT and AST by 1 h ischemia and reperfusion. Bcl-2 / B lymphocyte subsets ) Protein, bcl-2-related protein (bax) content, morphology was observed after hematoxylin-eosin staining, the proportion of necrotic hepatocytes was counted to evaluate the degree of injury. Results The serum levels of ALT, AST, necrotic cells and the levels of bcl-2 and bax protein in C2h, P2h, F2h, C4h, P4h and F4h subgroups were significantly higher than those in S2h and S4h subgroups (P <0.05) (P <0.05), and the levels of bcl-2 and bcl-2 in F4h, F4h and F4h subgroups were lower than those in C2h and C4h subgroups ). Conclusion Phosphofovir disoproxil sodium has a protective effect on hepatic ischemia-reperfusion injury, and its protective mechanism may be related to the regulation of bcl-2 and bax protein expression.