应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。 The purified Bt1 insecticidal crystal protein was used as a standard protein and an immunogen. The enzyme-linked immunosorbent assay (ELISA) was established to detect Bt1 protein in the transgenic maize by antibody-antigen-enzyme antibody reaction. The ELISA method was used to determine the four kinds of imported corn products. The experimental results were verified by Western blot analysis and consistent with the quantitative analysis results of the imported kit method. The established ELISA method is simple, rapid and specific, Quantitative, cost-effective advantages, especially suitable for high-volume testing, has a good prospect.