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5. CHEMICAL MODIFICATION OF SULFHYDRYL GROUPS OF E.coli LEUCYL-tRNA SYNTHE-TASE AND SEQUENCING OF [<

CHEMICAL MODIFICATION OF SULFHYDRYL GROUPS OF E.coli LEUCYL-tRNA SYNTHE-TASE AND SEQUENCING OF [<

来源 :中国科学：化学英文版 | 被引量 : 0次 | 上传用户：abenwin

【摘 要】

：

The chemical modification of the sulfhydryl groups of E. coli Leucyl--tRNA synthetase（LeuRS） by DTNB, NEM and IAA resulted in a time-dependent loss of both amino-

【作 者】

：

缪枫 施建平 王应睐 

【机 构】

：

ShanghaiInstituteojBiochemistry,AcademiaSinica,Shanghai200031,PRC,PRC

【出 处】

：

中国科学：化学英文版

【发表日期】

：

1991年6期

【关键词】

：

LeuRS chemical modification [¹⁴C]NEM-labeled PEPTIDE LeuR 

【基金项目】

：

Supported by the National Natural Science Foundation of China and the President’s Research Foundation of Academia Sinica.

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The chemical modification of the sulfhydryl groups of E. coli Leucyl--tRNA synthetase（LeuRS） by DTNB, NEM and IAA resulted in a time-dependent loss of both amino-acid acti-vation and aminoacylation activities in parallel. The second-order reaction constan

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