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目的:利用体外抗氧化模型,定量比较丹参药材中的主要水溶性成分丹参素、原儿茶醛、咖啡酸和丹酚酸B的体外清除超氧阴离子能力的强弱,并探讨其抗氧化能力产生的构效关系和量效关系,为寻找丹参治疗心血管疾病主要活性成分提供依据。方法:本实验采用黄嘌呤—黄嘌呤氧化酶反应,根据公式计算对超氧阴离子(O2-)的清除率,建立量效关系曲线,通过量效曲线算出清除率为50%时样品的浓度(IC50)值,IC50值反映了样品对超氧阴离子(O2-)的清除能力。结果:4种酚性化合物清除超氧阴离子的IC50值大小顺序为:丹参素>咖啡酸>原儿茶醛>丹酚酸B,表明丹酚酸B的作用最强,丹参素钠、原儿茶醛和咖啡酸的作用相近。结论:丹酚酸B可能是丹参通过清除氧自由基治疗心血管疾病的重要活性成分。
OBJECTIVE: To quantitatively compare the ability of the major water-soluble constituents Danshensu, protocatechuic aldehyde, caffeic acid and salvianolic acid B in scavenging superoxide anion in vitro and to investigate the antioxidant capacity of in vitro antioxidative models. The resulting structure-activity relationship and dose-effect relationship provide the basis for finding salvia miltiorrhiza in the treatment of cardiovascular diseases. METHODS: In this experiment, the xanthine-xanthine oxidase reaction was used to calculate the clearance rate of superoxide anion (O2-) according to the formula, and a dose-effect relationship curve was established. The concentration of the sample at a clearance rate of 50% was calculated from the dose-effect curve. IC50) value, IC50 value reflects the sample’s ability to remove superoxide anion (O2-). Results: The IC50 values of four phenolic compounds in scavenging superoxide anions were as follows: Danshensu> Caffeic acid> Protocatechuic aldehyde> Salvianolic acid B, indicating that salvianolic acid B had the strongest effect, Danshensu sodium, and protozoa The role of tea aldehyde and caffeic acid is similar. Conclusion: Salvianolic acid B may be an important active component of Salvia miltiorrhiza in the treatment of cardiovascular diseases by scavenging oxygen free radicals.