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核桃枝枯病是核桃树上的重要病害之一。在核桃树因受逆境影响而生长不良时,病害尤为严重。国内外对该病害的研究甚少,而且多半着重于对病害发生规律的一般调查和研究。本文报导矩园黑盘菌(Mel—anconiunm obongum Berk)的分离及其生物学特性的研究。材料和方法自然发病枝条采自北京林学院南门外15年生核桃树。用组织分离法和孢子稀释法分离。分离用培养基为PDA、PDA+10%核桃枝皮水提液(以下简称PDAB培养基)。茵丝生长测定用培养基为PDA、PDAB、玉米粉、查氏、高氏1号、黑盘孢菌产孢培养基、黑盘孢菌基本培养基等。孢子萌发试验用凹玻片液滴法。接种用孢子接种和菌
Walnut leaf blight is one of the important diseases on the walnut tree. The disease is particularly serious when walnut trees grow poorly as a result of adverse conditions. Domestic and foreign research on the disease is very little, and most of the emphasis on the law of disease occurrence of the general investigation and research. This article reports the isolation and biological characterization of Mel-anconiunm obongum Berk. Materials and Methods Naturally occurring shoots were collected from 15-year-old walnut trees outside the south gate of Beijing Forestry College. Isolation by tissue and spore dilution method. Separation medium for PDA, PDA + 10% walnut water extract branch (hereinafter referred to as PDAB medium). Inosine growth assay medium for the PDA, PDAB, corn meal, Cha’s, Gao’s No. 1, black spore spore culture medium, Spirulina basic medium and so on. Spore germination test with concave glass droplet method. Inoculation with spore inoculation and bacteria