论文部分内容阅读
分别以旱芹（ＡＰｉｕｍｇｒａｖｅｏｌｅｎｓ）的天然种子、子叶、真叶等为外植体，在ＭＳ＋２，４－Ｄｌｍｇ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ的培养基上诱导产生胚状体包埋成的人工种子，可获得最高的转株率和最低的畸形率。四种愈伤组织的ＳＤＳ－ＰＡＧＥ电泳结果表明，子叶愈伤组织的蛋白成分比其它三种愈伤组织缺少８６ｋＤ、６３ｋＤ、１７ｋＤ、１５ｋＤ、１３ｋＤ的蛋白，这可能决定了子叶胚状体的高质量。另外，用ＭＳ＋ＡＢＡ１ｍｇ／Ｌ处理子叶愈伤组织，可使胚状体的蛋白含量从１．３ｍｇ／ｇ（鲜重）提高到２．０ｍｇ／ｇ，从而大大提高了人工种子冷藏后的转株率。所以，胚状体的优化是必要的，也是可行的。
Artificial seeds embedded in embryoid bodies were induced on the medium of MS + 2,4-Dlmg / L + KT0.5mg / L using natural seeds, cotyledons and leaves of Apium graveolens as explants, respectively Get the highest transplanted plant rate and the lowest deformity rate. SDS-PAGE of the four callus showed that the protein content of cotyledons lacked 86kD, 63kD, 17kD, 15kD and 13kD proteins compared with the other three callus, which may determine the cotyledon embryoid body height quality. In addition, treatment of cotyledons with MS + ABA 1 mg / L increased the protein content of embryoid body from 1.3 mg / g (fresh weight) to 2.0 mg / g, thus greatly increasing the rate of transplanted plants after artificial storage . Therefore, the optimization of embryoid bodies is necessary and feasible.