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喇曼散射光谱是一种测定物质组分和结构的重要方法,它与红外光谱方法是互相补充的,两者的配合可提供更丰富的信息,由于自发喇曼散射的强度较弱,使对微量成分的测定存在困难。1974年表面增强喇曼散射现象(Surface Enhanced Raman scattering—SERS)的发现,使喇曼散射光强度比常规喇曼散射光强度增加10~5—10~7倍,对痕量分析的应用有重要意义,因此受到广泛重视。银表面的喇曼增强强度是最大的,目前基本上包括有三种方式:第一种是银电极表面增强,第二种是银胶溶液增强,第三种是基底上镀银膜的表面增强,银电极方法在电极使用后必须通过氧化还原法进行再生;银胶则必须与样品均匀混合,而且增强强度将随时间而变化;基底上镀银膜的表面增强制备并不复杂,可以保存一定时间,通过严格控制条件,可得到基底粒子分布和银膜蒸镀均匀的表面,达到较好的表面增强重现性,在此基础上,可能发成为用于流动样品检测的手段,如液相色谱检测器等。
Raman scattering spectroscopy is an important method for determining the composition and structure of matter. It is complementary to the infrared spectroscopy method. The combination of the two can provide richer information. Due to the weak intensity of spontaneous Raman scattering, The determination of trace elements is difficult. The 1974 discovery of Surface Enhanced Raman scattering (SERS) has led to an increase in Raman scattering light intensity of 10-5-10-7 times the conventional Raman scattering light intensity, which is important for the application of trace analysis Meaning, it has received widespread attention. Raman enhancement of silver surface intensity is the largest, currently basically includes three ways: the first is the silver electrode surface enhancement, the second is enhanced silver glue solution, the third is the silver-coated substrate surface enhancement, The silver electrode method must be regenerated by the redox method after the electrode has been used; the silver paste must be homogeneously mixed with the sample and the intensity of the increase will change over time; the surface-enhanced preparation of the silver-plated film on the substrate is not complicated and can be preserved for a certain period of time , Through the strict control conditions, the base particle distribution and silver film evaporation uniform surface, to achieve better surface enhanced reproducibility, on the basis of which may be made for the flow of sample detection means, such as liquid chromatography Detector and so on.