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采用免疫组化的方法，选取地塞米松和Ｐ物质分别代表内分泌和神经因素，研究了它们单独作用及共同作用时对Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应性的影响，并用显微图像分析系统进行相对定量分析处理；同时用热板测痛法测量了痛阈的变化。结果表明：①地塞米松尾静脉注射后痛阈升高，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应减弱，提示地塞米松可能有抑制痛觉信息一级传入的作用；②Ｐ物质鞘内注射后痛阈降低，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应增强，Ｐ物质可能有促进痛觉信息一级传入的作用；③地塞米松和Ｐ物质共同作用后痛阈有所降低，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应强度介于二物质单独作用之间，似乎二者的作用发生了“中和”，但免疫反应仍较对照组增强。结果提示：痛觉的一级传入可能受神经－内分泌相互作用的影响。
Immunohistochemical method was used to determine the effect of dexamethasone and substance P on endocrine and neurological factors respectively. The effects of these two drugs on the immunoreactivity of CGRP in lamina I and II of L4 and L5 spinal ganglia and spinal dorsal horn , And the relative quantitative analysis was carried out by the microscopic image analysis system. Meanwhile, the change of pain threshold was measured by the hot plate method. The results showed that: (1) After the injection of dexamethasone tail vein, the pain threshold increased, the CGRP immunoreactivity in the L4, L5 spinal ganglia and spinal dorsal horn Ⅰ and Ⅱ layers decreased, suggesting that dexamethasone may inhibit the primary afferent of the pain sensation information; ② After intrathecal injection of substance P, the pain threshold decreased, the CGRP immunoreactivity increased in the L4 and L5 spinal ganglia and the spinal cord posterior horn Ⅰ and Ⅱ, and substance P may promote the primary afferent of the pain information. ③ Dexamethasone and substance P together After the action of pain threshold decreased, L4, L5 spinal ganglia and spinal dorsal horn Ⅰ, Ⅱ CGRP immune response intensity between the two substances alone, it seems that both the role of “neutralization”, but the immune response is still Compared with the control group. The results suggest that primary afferent pain may be affected by neuroendocrine interactions.