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[目的]探讨在Ku80 mRNA双链小RNA片段有效抑制A549肺癌细胞Ku80表达的基础上联合放疗增强肿瘤组织放射敏感性的可行性。[方法]体外培养A549肺癌细胞,并制备BALB/c裸鼠实体瘤模型,用Ku80 siRNA转染肿瘤组织。用免疫组织化学法测定转染Ku80 siRN A48h后肿瘤组织内Ku80蛋白表达情况。Ku80 siRNA转染BALB/c裸鼠实体瘤48h后进行局部射线照射10Gy。隔日测量一次肿瘤体积,共观察20d,绘制肿瘤生长曲线,观察肿瘤体积、相对生长速率和生长抑制率。[结果]转染Ku80 siRNA后移植瘤内Ku80蛋白表达量较各对照组减少(P<0.05),而且转染Ku80 siRNA并联合射线照射组的肿瘤体积、相对生长速率和生长抑制率与各对照组比较,差异均有统计学意义(P=0.000)。[结论]转染Ku80 siRNA后A549肺癌细胞Ku80蛋白表达下调,放射敏感性增强。
[Objective] The purpose of this study was to investigate the feasibility of radiosensitization of tumor tissue enhanced by combination of radiotherapy and Ku80 mRNA double-stranded RNA of Ku80 effectively inhibiting Ku80 expression in A549 lung cancer cells. [Method] A549 lung cancer cells were cultured in vitro, and the solid tumor model of BALB / c nude mice was prepared. The tumor tissues were transfected with Ku80 siRNA. The expression of Ku80 protein in Ku80 siRN A48h cells was detected by immunohistochemistry. Ku80 siRNA transfection of BALB / c nude mice solid tumors after 48h local irradiation 10Gy. Tumor volume was measured every other day for a total of 20 days. The tumor growth curve was drawn and the tumor volume, relative growth rate and growth inhibition rate were observed. [Results] The expression of Ku80 protein in the transplanted tumor was decreased compared with the control group (P <0.05) after transfected with Ku80 siRNA. The tumor volume, relative growth rate and growth inhibition rate of Ku80 siRNA combined with radiation group were significantly decreased The differences between the two groups were statistically significant (P = 0.000). [Conclusion] The expression of Ku80 protein in A549 lung cancer cells transfected with Ku80 siRNA is down-regulated and the radiation sensitivity is enhanced.