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目的:研究γ-氨基丁酸A(GABAA)受体与七氟烷(S)致幼小鼠遗忘作用和自主活动抑制作用的关系。方法:取幼小鼠随机分为3大组(n=80),分别进行自主活动、跳台和避暗实验,再将每大组幼小鼠随机分为空白对照组(生理盐水10ml/kg),S组(3.0ml/kg),GABAA受体特异性阻滞药一叶萩碱(Se)低、中、高剂量组(2、4、8mg/kg)及其联用低、中、高剂量组(S3.0ml/kg+Se2、4、8mg/kg),每组10只。实验开始时予幼小鼠腹腔注射Se或生理盐水,10min后皮下注射S,记录末次给药后15、30、45、60min时自主活动实验幼小鼠5min内的自主活动次数,末次给药后1、2d时跳台和避暗实验步入潜伏期、跳下潜伏期和错误次数。结果:与空白对照组比较,S组幼小鼠给药后15、30、45min时的自主活动次数明显减少,给药后1d的步入潜伏期、跳下潜伏期均明显缩短,其相应错误次数均明显增加(P<0.05);联用低、中、高剂量组幼小鼠给药后15min时的自主活动次数明显减少(P<0.05)。与S组比较,联用高剂量组幼小鼠给药后1d的步入潜伏期、跳下潜伏期均明显延长,其相应错误次数均明显减少(P<0.05)。其余各组指标间差异无统计学意义(P>0.05)。结论:GABAA受体参与了S的遗忘作用,但S对自主活动的抑制作用与GABAA受体无关。
Objective: To investigate the relationship between amnesic and autonomic inhibitory activity of γ-aminobutyric acid A (GABAA) receptor and sevoflurane (S) -induced young mice. Methods: The young mice were randomly divided into three groups (n = 80). The mice were randomly divided into control group (saline 10ml / kg), S Group (3.0ml / kg), GABAA receptor specific blockers of low, medium and high doses of selenium (2, 4, 8mg / kg) and low, medium and high dose (S3.0ml / kg + Se2,4,8mg / kg), each group of 10. At the beginning of the experiment, the mice were intraperitoneally injected with Se or saline, injected with S subcutaneously 10 minutes later, and recorded the number of spontaneous activities within 5 minutes after the last administration at 15, 30, 45 and 60 minutes. After the last administration, 2d jumping and avoiding darkness experiment into the incubation period, jumped off the incubation period and the number of errors. Results: Compared with the blank control group, the number of spontaneous activity decreased significantly at 15, 30, 45 min after administration in the S group, and the latency to entry and the latency to jump off one day after administration were significantly shortened, and the corresponding errors were significant (P <0.05). The number of spontaneous activity decreased significantly at 15 min after administration in low, medium and high dose groups (P <0.05). Compared with the S group, the latency of the mice in the combined high-dose group on the 1st day after administration increased significantly, and the corresponding error times were significantly reduced (P <0.05). There was no significant difference between other groups (P> 0.05). CONCLUSION: GABAA receptor is involved in the amnesia of S, but the inhibitory effect of S on autonomic activity is not related to GABAA receptor.