【摘 要】
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In this study,orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolac-tone(PCL)scaffolds by the near-field direct-writing melt electro-spinning(NFDWMES)technology.Based on the optimal process pa-rameters,t
【机 构】
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School of Textile Science and Engineering,State Key Laboratory of Sepa-ration Membranes and Membrane
论文部分内容阅读
In this study,orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolac-tone(PCL)scaffolds by the near-field direct-writing melt electro-spinning(NFDWMES)technology.Based on the optimal process pa-rameters,the PCL scaffolds with different thicknesses,gaps and structures were manufactured and the corresponding hydrophilici-ties were characterized.The PCL scaffolds were modified by chitosan(CS)and hyaluronic acid(HA)to improve biocompatibility and hydro-philicity.Both Fourier transform infrared spectroscopy(FTIR)analy-sis and antibacterial experimental results show that the chitosan and hyaluronic acid adhere to the surface of PCL scaffolds,sugges-ting that the modification plays a positive role in biocompatibility and antibacterial effect.The PCL scaffolds were then employed as a carrier to culture cells.The morphology and distribution of the cells observed by a fluorescence microscope demonstrate that the mo-dified PCL scaffolds have good biocompatibility,and the porous structure of the scaffolds is conducive to adhesion and deep growth of cells.
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