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目的检测丙酮酸羧化酶(PC)在胃腺癌组织中的表达并分析其临床意义。方法应用免疫组织化学方法及Westernblot法检测PC蛋白在胃腺癌及其对应癌旁组织的表达情况,分析其在肿瘤中表达水平与患者年龄、性别、TNM分期及病理分级等临床病理资料之间的关系,并用Western blot法检测PC蛋白在不同分化胃癌细胞系及永生化胃黏膜上皮细胞系的表达情况。结果免疫组化及Western blot法均显示PC在胃腺癌组织表达水平显著高于其对应癌旁组织(P<0.01)。PC高表达与患者TNM分期及病理分级显著相关(P<0.05),而与患者性别、年龄无明显相关性(P>0.05)。Western blot法显示PC在不同分化胃癌细胞系表达水平显著高于永生化胃黏膜上皮细胞(P<0.01)。结论 PC高表达与肿瘤的恶性进展密切相关,提示PC有可能作为胃癌生物治疗的潜在靶点。
Objective To detect the expression of pyruvate carboxylase (PC) in gastric adenocarcinoma and to analyze its clinical significance. Methods The expression of PC protein in gastric adenocarcinoma and corresponding paracancerous tissues was detected by immunohistochemistry and Western blotting. The relationship between PC protein expression and clinicopathological parameters such as age, gender, TNM stage and pathological grading were analyzed Western blot was used to detect the expression of PC protein in different differentiated gastric cancer cell lines and immortalized gastric mucosal epithelial cell lines. Results Both immunohistochemistry and Western blot showed that the expression of PC in gastric adenocarcinoma was significantly higher than that in paracancerous tissues (P <0.01). The high expression of PC was significantly associated with TNM staging and pathological grade (P <0.05), but not with the gender and age of patients (P> 0.05). Western blot showed that the expression level of PC in gastric cancer cell lines with different differentiation was significantly higher than that of immortalized gastric epithelial cells (P <0.01). Conclusion The high expression of PC is closely related to the malignant progression of the tumor, suggesting that PC may be a potential target for biotherapy of gastric cancer.