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[目的 ]研究广藿香挥发油 (B)与青蒿酯钠 (SA)联合用药对伯氏疟原虫 (P.b)正常株 (N)及抗青蒿酯钠株 (R)的抗疟增效作用和对 R的逆转抗性作用。[方法 ]采用 4天抑制试验法对分别感染 N和 R小鼠进行抗疟实验 ,以两药等效剂量配伍 ,用直线回归法计算其抑制原虫 5 0 %的药物剂量 (SD5 0 )。[结果 ]B单独用药 ,N:SD5 0 =87.6 4± 19.5 8(GKD) ,R:SD5 0 =43.2 4± 7.71(GKD) ;SA单独用药 ,N:SD5 0 =0 .88± 0 .0 1(MGKD) ,R:SD5 0 =2 7.6 9± 0 .93(MGKD)。B与 SA联合用药 ,N:B的 SD5 0 =36 .89± 4.5 7(GKD) ,SA的 SD5 0 =0 .39± 0 .0 5 (MGKD) ;R的 SD5 0 =7.40± 1.30 (GKD) ,SA的 SD5 0 =4.2 1± 0 .74(MGKD)。联合用药 B对 SA的增效指数 ,N、R分别为 2 .2和 6 .6 ,B对 R的逆转抗性倍数为 6 .6。相对逆转率为 87.6 %。[结论 ]采用 B和 SA联合用药对 P.b的抑制作用具有增效作用 ,能逆转 R对 SA的抗性及延缓 N对 SA抗性的发生。
[Objective] The research aimed to study the anti-malarial synergism effect of combined application of volatile oil of Pogostemon cablin (B) and artesunate sodium (SA) on normal strain of Plasmodium berghei (P) and anti-artemisinin sodium (R) And reversal of R resistance. [Method] The 4-day inhibition test was used to test malaria resistance in mice infected with N and R respectively. The drug dose (50% inhibition) of protozoal was calculated by linear regression with the equivalent dose of two drugs. [Results] B alone, N: SD5 0 = 87.6 4 ± 19.5 8 (GKD), R: SD5 0 = 43.2 4 ± 7.71 (GKD); SA alone, N: SD5 0 = 0.88 ± 0 .0 1 (MGKD), R: SD5 0 = 2 7.69 ± 0.93 (MGKD). B with SA, SD5 of N: B = 36.89 ± 4.57 (GKD), SA of SD5 0 = 0.39 ± 0.05 (MGKD); R of SD5 0 = 7.40 ± 1.30 (GKD ), SD5 0 of SA = 4.2 1 ± 0 .74 (MGKD). The synergistic index of the combination of B with SA, N, R were 2.2 and 6.6, respectively, and the ratio of B to R was -6.6. The relative reversal rate was 87.6%. [Conclusion] The combination of B and SA could inhibit the inhibition of P.b, which could reverse the resistance of R to SA and delay the occurrence of N against SA.