论文部分内容阅读
目的:通过红景天苷对模拟航渡并高强度运动大鼠的干预,观察干预后大鼠下丘脑-垂体-性腺(HPG)轴的功能及超微病理学的改变。方法:将6周龄的SD雄性大鼠随机分为非应激对照组(NS组,n=10)、训练对照组(TC组,n=12)和红景天苷干预组(ST组,n=12)。NS组不接受任何刺激,在生理盐水灌胃1周后即进行取样。TC组和ST组大鼠经过10d递增负荷跑台训练,分别用生理盐水和红景天苷灌胃1周后,进行模拟航渡及高强度运动,结束即刻取血,使用放免法测定大鼠血清睾酮(T)、皮质酮(CORT)、促肾上腺皮质激素(ACTH)、促黄体生成素(LH)和促性腺激素释放激素(GnRH);HE染色观察睾丸组织病理结构;电镜下观察垂体、睾丸超微结构。结果:①TC组大鼠与NS组相比,血清T水平显著下降;灌服红景天苷后与TC组相比,血清T水平显著增高,与NS组比较差异无统计学意义;②HE染色示3组大鼠的睾丸组织病理无明显变化;③电镜检查示TC组垂体细胞胞浆中分泌颗粒显著减少,而ST组与TC组相比胞浆中分泌颗粒的数目增多明显;TC组睾丸Leydig细胞线粒体肿胀,电子密度增高,嵴减少或消失,ST组睾丸Leydig细胞中见线粒体结构趋向于正常,嵴大多数清晰,可见大小不等深染的分泌颗粒。结论:负性心理应激及高强度的运动训练会抑制HPG轴的功能,灌服红景天苷对HPG轴具有保护作用。
OBJECTIVE: To investigate the effects of salidroside on rats undergoing simulated high-intensity exercise and to observe the changes in hypothalamic-pituitary-gonadal (HPG) axis and ultrastructural pathology after intervention. METHODS: Six-week-old SD male rats were randomly divided into non-stress control group (NS group, n=10), training control group (TC group, n=12) and salidroside intervention group (ST group, n=12). The NS group received no stimulation and was sampled 1 week after intragastric administration. Rats in TC group and ST group were trained with 10 days of incremental load treadmill. After normal saline and salidroside were intragastrically administered for 1 week, simulated gliding and high-intensity exercise were performed. Blood was taken immediately and rats were measured by radioimmunoassay. Serum testosterone (T), corticosterone (CORT), adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), and gonadotropin-releasing hormone (GnRH); HE staining was used to observe the pathological structure of testicular tissue; pituitary was observed under electron microscope. Testicular ultrastructure. RESULTS: Compared with NS group, serum T levels in TC group were significantly lower than those in NS group. Compared with TC group, serum T level was significantly increased in TC group compared with NS group. There was no significant difference between TC group and NS group. There was no significant change in pathology of the testis in the three groups.3 Electron microscopy showed that secretory granules in the cytoplasm of the pituitary cells in the TC group were significantly reduced, while the numbers of secretory granules in the cytoplasm of the ST group were significantly increased compared with the TC group; Leydig was found in the TC group. The mitochondria of the cells swelled, the electron density increased, and the hernias decreased or disappeared. The mitochondrial structure in the Leydig cells of the ST group tended to be normal, and most of the hernias were clear, and secreted granules of varying sizes were seen. Conclusion: Negative psychological stress and high-intensity exercise training can inhibit the function of HPG axis, and salidroside can protect HPG axis.