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目的:探讨高迁移率族蛋白B1(HMGB1)对于大鼠骨髓基质干细胞BMSCs增殖、成骨/破骨的影响。方法:原代培养的大鼠BMSCs,分别以浓度为0,100,500 ng/mL HMGB1作用于BMSCs,MTT法检测细胞数目;以浓度为0,100,500 ng/mL HMGB1作用于BMSCs,7d后行ALP染色和RT-PCR检测成骨/破骨基因的表达(骨钙素OCN,骨保护素OPG,核因子κB受体活化因子配体RANKL)。结果:HMGB1在100、500 ng/mL浓度时,第3 d和第5 d BMSCs的OD值有明显升高,在第7 d对BMSCs的ALP染色没有明显的改变,OCN和OPG基因的表达也没有明显变化,HMGB1明显提高了BMSCs的RANKL及RANKL/OPG基因表达。结论:HMGB1对大鼠BMSCs的增殖和破骨基因表达有明显的促进作用,为HMGB1应用于临床提供实验依据。
Objective: To investigate the effect of high mobility group box 1 protein (HMGB1) on the proliferation, osteogenesis and osteoclast formation of BMSCs in rat bone marrow stromal cells. METHODS: Primary cultured rat BMSCs were treated with HMGB1 at concentrations of 0, 100 and 500 ng / mL, respectively. BMSCs were treated with HMGB1 at concentration of 0, 100, and 500 ng / mL HMGB1 for 8 days. ALP staining and RT-PCR Osteogenetic / osteoclast gene expression (osteocalcin OCN, osteoprotegerin OPG, nuclear factor kappa B receptor activator ligand RANKL) was examined. Results: At the concentration of 100,500 ng / mL, the OD value of BMSCs at the 3rd and 5th day of HMGB1 significantly increased. At the 7th day, there was no significant change in the ALP staining of BMSCs. The expressions of OCN and OPG No significant change, HMGB1 significantly increased RANKL and RANKL / OPG gene expression in BMSCs. CONCLUSION: HMGB1 can significantly promote the proliferation and osteoclast gene expression of BMSCs in rats, providing an experimental basis for the clinical application of HMGB1 in clinic.