目的：评价微量组织培养半数感染量病毒滴定法（简称微量ＴＣＩＤ５０法）检测４种常用消毒剂对脊髓灰质炎病毒（ｐｏｌｉｏＶ）的灭活效果。方法：将ＰｏｌｉｏＶ滴定于９６孔细胞培养板中的细胞上进行培养，测定病毒接触消毒剂前后感染细胞的滴度。结果：２％碱性戊二醛、１％二氯异氰尿酸钠（５２００ｍｇ·Ｌ１）５ｍｉｎ内能有效杀灭ｐｏｌｉｏＶ（病毒滴度下降≥３ｌｏｇ），０．２％过氧乙酸和０．５％碘伏（４０００ｍｇ·Ｌ１）需１０ｍｉｎ（病毒滴度下降＞３ｌｏｇ）。结论：用微量ＴＣＩＤ５０法评价消毒剂对病毒的灭活效果，客观、灵敏、简便易行 OBJECTIVE: To evaluate the inactivation effect of four commonly used disinfectants against polioV by microtiter microdialysis virus titration (TCID50 for short). Methods: PolioV was titrated on the cells in 96-well cell culture plates and the titer of infected cells before and after exposure to disinfectant was measured. Results: 2% alkaline glutaraldehyde, 1% sodium dichloroisocyanurate (5200mg · L1) within 5min can effectively kill polioV (viral titer drop ≥ 3log), 0.2% peracetic acid and 0 .5% iodophor (4000mg · L  1) takes 10min (viral titer drop> 3log). Conclusion: TCID50 method was used to evaluate the inactivation effect of disinfectant on virus, objective, sensitive and simple