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建立了 HPLC测定人血浆及尿中盐酸氨溴索含量的方法 :Hypersill C18柱 (4.6mm× 2 50 mm,5μm) ,乙腈 -甲醇 - 0 .0 1 mol/L磷酸盐缓冲液 -四氢呋喃 (35∶ 35∶ 2 7.5∶ 2 .5,V/V)为流动相 ,流速 1 .5ml/min,检测波长 2 4 2 nm。结果表明 :最低检测浓度为 5ng/ml,血药浓度在 1 0～ 32 0 ng/ml范围内线性良好 ,尿药浓度在 0 .2 5～ 8.0μg/ml范围内线性良好 ,氨溴索生物半衰期为 (4.2 1± 0 .93) h。
A HPLC method was established for the determination of ambroxol hydrochloride in human plasma and urine. Hypersill C18 column (4.6 mm × 250 mm, 5 μm), acetonitrile-methanol-0.1 mol / L phosphate buffer-tetrahydrofuran : 35: 2 7.5: 2.5, V / V) as the mobile phase at a flow rate of 1.5 ml / min with a detection wavelength of 224 nm. The results showed that the lowest detection concentration was 5ng / ml and the linear range was 10 ~ 32 0 ng / ml. The linearity of urinary drug concentration was in the range of 0.52 ~ 8.0μg / ml. The concentration of ambroxol The half-life was (4.2 1 ± 0.93) h.