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目的:采用荧光分析法检测血液中微量多巴胺的浓度,为临床治疗调整用药剂量提供依据。方法:通过加入荧光发生剂碘与亚硫酸钠,与DA产生氧化还原反应生成三羟基吲哚,发生特异性荧光。在激发波长327nm、发射波长368.4nm处测定荧光强度,建立多巴胺一定范围内浓度与荧光强度的线性关系,用于检测多巴胺的血药浓度。结果:盐酸多巴胺浓度在1～10μg/mL范围内的吸收度与浓度呈良好的线性关系,而临床常用剂量下血液中多巴胺浓度可落在此线性范围之内。结论:荧光分析法可用于检测盐酸多巴胺的微量血药浓度。
OBJECTIVE: To detect the concentration of trace dopamine in the blood by fluorescence analysis and provide the basis for adjusting the dosage of the drug for clinical treatment. Methods: Iodine and sodium sulfite were added to produce trihydroxyindole by DA reaction with DA to generate specific fluorescence. Fluorescence intensity was measured at excitation wavelength of 327nm and emission wavelength of 368.4nm to establish a linear relationship between concentration and fluorescence intensity of dopamine within a certain range for the determination of dopamine plasma concentration. Results: There was a good linear relationship between the concentration of dopamine hydrochloride and the concentration of dopamine hydrochloride in the range of 1 ~ 10μg / mL. However, the concentration of dopamine in the blood could fall within this linear range at the commonly used dose of dopamine. Conclusion: Fluorescence analysis can be used to detect trace concentrations of dopamine hydrochloride.