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观察施万细胞(SCs)对骨髓基质细胞(BMSCs)的诱导分化作用。分离和体外培养SD大鼠SCs和BMSCs,分为SCs+BMSCs共培养(实验组)和BMSCs单独培养(对照组)。用流式细胞仪(FCM),S100、Brdu/nestin、Brdu/TH免疫荧光法分别鉴定BMSCs、SCs和BMSCs的分化情况。第2代SCs呈S100阳性,第3代BMSCs呈CD29和CD90阳性,CD45阴性。二者共培养3d后,可见Brdu/nestin免疫荧光双标细胞,阳性率达28.3±1.3%,与对照组比较,P<0.05。7d后,Brdu/nestin双标细胞减少,出现Brdu/TH免疫荧光双标细胞,阳性率为19.2±1.6%,与对照组比较,P<0.05。而对照组始终只见Brdu单标细胞。上述结果表明SCs可诱导共培养的BMSCs分化为DA能神经元。
To observe the differentiation of bone marrow stromal cells (BMSCs) induced by Schwann cells (SCs). The SCs and BMSCs of SD rats were isolated and cultured in vitro. They were co-cultured with SCs + BMSCs (experimental group) and BMSCs alone (control group). The differentiation of BMSCs, SCs and BMSCs were respectively identified by flow cytometry (FCM), S100, Brdu / nestin and Brdu / TH immunofluorescence. The second generation SCs were S100 positive, the third generation of BMSCs positive for CD29 and CD90, CD45 negative. After co-cultured for 3 days, the positive rate of Brdu / nestin immunofluorescence double labeled cells was 28.3 ± 1.3%. Compared with the control group, the Brdu / nestin double-labeled cells decreased after P <0.05. Fluorescent double labeled cells, the positive rate was 19.2 ± 1.6%, compared with the control group, P <0.05. The control group only see Brdu single-labeled cells. The above results indicate that SCs can induce co-cultured BMSCs to differentiate into DA neurons.