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目的:观察养生保健1号对慢性不可预知温和应激刺激抑郁大鼠学习记忆能力相关的脑神经递质及胆碱能系统的调节作用。方法:SD大鼠随机分为正常组,模型组,保健方高、中、低剂量组(7.26、4.84、3.23g/kg),除正常组外建立慢性不可预知应激温和刺激抑郁模型(CUMS)。采用旷场实验、糖水消耗实验评价大鼠行为学变化,采用Morris水迷宫考察CUMS大鼠的学习记忆能力,同时测定大脑神经递质5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)水平及乙酰胆碱(Ach)、Ach E含量变化。结果:应激刺激3周,与正常组比较,大鼠糖水偏嗜度显著下降(P<0.05)。养生保健方各剂量组糖水的偏嗜度较模型组明显增加(P<0.01)。旷场实验中,模型组大鼠运动的总得分低于正常组(P<0.01),保健方各个剂量组的运动总路程均明显高于模型组(P<0.05)。Morris水迷宫定位导航实验中,模型组大鼠平均潜伏期长于正常组(P<0.05),与模型组比较,保健方各剂量组的潜伏期缩短(P<0.05)。Morris水迷宫空间探索实验中,模型组大鼠的穿越平台次数、原台所在象限游泳距离和时间明显少于正常组(P<0.05,P<0.01),而保健方各剂量组较模型组均明显增加(P<0.01)。与正常组比较,模型组脑内5-HT、NE、Ach降低Ach E升高;与模型组比较,保健方高、中剂量组大鼠5-HT含量显著增加(P<0.05,P<0.01),保健方中剂量组NE含量显著升高(P<0.05)。保健方3个剂量组大鼠脑浆上清中Ach含量均显著升高(P<0.05),而Ach E含量明显降低(P<0.05)。结论:养生保健1号对CUMS大鼠具有抗抑郁、改善学习障碍、提高空间学习记忆能力的作用,其机制与其对大鼠脑单胺类神经递质5-HT、NE及Ach的上调有关。
OBJECTIVE: To observe the regulative effects of “Health-Preserving Health No.1” on brain neurotransmitter and cholinergic system related to learning and memory abilities in rats with chronic unpredictable and mild stress. Methods: SD rats were randomly divided into normal group, model group, high, medium and low dose group (7.26,4.84,3.23g / kg), except for normal group, chronic unpredictable mild stress depression model (CUMS ). The open field test and water consumption test were used to evaluate the behavioral changes in rats. Morris water maze was used to investigate the learning and memory abilities of rats with CUMS. The levels of neurotransmitter 5-hydroxytryptamine (5-HT), dopamine (DA) Epinephrine (NE) levels and acetylcholine (Ach), Ach E content changes. Results: After 3 weeks of stress stimulation, compared with the normal group, the degree of partiality of glucose and water in rats significantly decreased (P <0.05). Compared with the model group, the degree of partiality of syneresis in each dose group of health care prescription increased significantly (P <0.01). In the open-field test, the total score of exercise in model group was lower than that in normal group (P <0.01), and the total exercise in each dose group was significantly higher than that in model group (P <0.05). Morris water maze positioning and navigation experiments, the average latency of the model group rats longer than the normal group (P <0.05), compared with the model group, the health side of each dose group latency (P <0.05). In Morris water maze space exploration experiment, the number of crossing platform and the swimming distance and time in the quadrant of the model group were significantly less than those in the normal group (P <0.05, P <0.01) Significantly increased (P <0.01). Compared with the normal group, 5-HT, NE, Ach in the model group decreased AchE, and compared with the model group, the content of 5-HT in the high and middle dose group increased significantly (P <0.05, P <0.01 ), And the NE content in the middle-dose of health prescription group was significantly increased (P <0.05). The content of Ach in the brain tissue of supernatant of the three prescriptions of Health Prescriptions were significantly increased (P <0.05), while the content of Ach E was significantly decreased (P <0.05). CONCLUSION: The effect of health-care-1 on CUMS rats is antidepressant, ameliorating learning disability and improving spatial learning and memory. Its mechanism is related to the upregulation of monoamine neurotransmitters 5-HT, NE and Ach in rats.