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目的 观察早孕绒毛、蜕膜形态学及雌孕激素受体的表达 ,对复方米非司酮及米非司酮进行比较性研究。 方法 4 9例早孕者随机分为对照、米非司酮 (15 0mg/次 )及复方米非司酮 (含双炔失碳酯 5mg +米非司酮 30mg ,12h× 2 ) 3组。服药后 2 4h手术终止妊娠 ,取绒毛、蜕膜组织 ,应用链霉卵白素 过氧化物酶 (SP)免疫组织化学法观察绒毛、蜕膜雌激素受体(ER)、孕激素受体 (PR)的变化。 结果 米非司酮组少数绒毛间质水肿变性 ,蜕膜示小灶性变性、坏死 ,可见凋亡细胞 ;绒毛ER、PR着染强度及阳性细胞着染百分率较对照组明显增加 (P <0 .0 0 1,P <0 .0 5 ) ,蜕膜ER、PR染色强度与对照组比较无显著差异 ,但阳性细胞着染百分率显著下降 (P <0 .0 5 ) ;复方米非司酮组绒毛表面附多量纤维蛋白样物 ,部分间质水肿 ,蜕膜呈片状变性、坏死 ,凋亡细胞散在 ;绒毛ER、PR着染强度较对照组显著增加 (P <0 .0 5 ) ,阳性细胞着染百分率与对照组比较ER显著增加 (P <0 .0 5 ) ,PR略低但无显著差异 ;蜕膜ER、PR着染强度无显著差异 ,但阳性细胞着染百分率与对照组比较明显下降(P <0 .0 0 1)。 结论 复方米非司酮使蜕膜ER、PR阳性细胞着染百分率明显降低 ,蜕膜变性、坏死较甚 ,但对ER、PR着染强度的影响相对小 ,这可能有助于提
Objective To observe the morphology of villi and decidua in early pregnancy and the expression of estrogen and progesterone receptor, and to compare the efficacy of mifepristone and mifepristone. Methods Forty-nine pregnant women with early pregnancy were randomly divided into control group, mifepristone group (15 0mg / time) and compound mifepristone (5mg / mifepristone 30mg, 12h × 2) group. Twenty-four hours after the operation, the pregnancy was terminated, and the villi and decidua were removed. The expression of ER, PR and PR were detected by immunohistochemistry with streptavidin peroxidase (SP) )The change. Results In the mifepristone group, a few of villus edema were degenerated, and decidual cells showed small focal degeneration and necrosis. Apoptotic cells were observed. The staining intensity of ER, PR and the percentage of positive cells in control group were significantly increased (P <0.05). The staining intensity of decidual ER and PR was not significantly different from that of the control group, but the percentage of staining of positive cells was significantly decreased (P <0.05); the compound mifepristone group Villus surface with a large number of fibrin samples, part of the interstitial edema, decidual degeneration, necrosis, apoptotic cells scattered; villi ER, PR staining intensity significantly increased compared with the control group (P <0.05), positive The percentage of staining of cells was significantly higher than that of the control group (P <0.05), while PR was slightly lower but no significant difference. There was no significant difference in the staining intensity of decidual ER and PR, but the staining percentage of positive cells compared with the control group Decreased significantly (P <0. 001). Conclusion Compound mifepristone can significantly reduce the percentage of ER and PR-positive cells in decidua, degeneration and necrosis of decidua, but have a relatively small influence on the staining intensity of ER and PR, which may be helpful to improve