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目的 观察反义增生细胞核抗原 (PCNA)和反义 bcl-2脱氧寡核苷酸对培养的人视网膜色素上皮细胞 (RPE)生长的作用 .方法 用 DNA合成仪合成有 1 8个核苷酸的反义PCNA和 1 5个核苷酸的反义 bcl- 2脱氧寡核苷酸片段 ,以不同浓度加入 RPE培养液 ,培养 2～ 4d,用 SABC法检测细胞PCNA和 bcl- 2的表达 ,用 MTT法及流式细胞仪测定细胞抑制率 .结果 6 .2 5～ 1 0 0μm ol· L- 1 的反义 PCNA对 RPE的抑制率为 8.3%～ 38.4% ,呈剂量依赖性 ,并抑制细胞 PCNA的表达 .反义 bcl- 2未显示抑制作用 .结论 反义 PCNA脱氧寡核苷酸可抑制近 40 %的 RPE生长 ,其临床应用值得进一步研究
Objective To observe the effects of antisense proliferating cell nuclear antigen (PCNA) and antisense bcl-2 deoxyribonucleotides on the growth of cultured human retinal pigment epithelial cells (RPE) .Methods DNA synthesizer was used to synthesize Antisense PCNA and antisense bcl-2 deoxyribonucleotide oligonucleotide fragment of 15 nucleotides were added into RPE culture medium at different concentrations and cultured for 2 to 4 days. The expression of PCNA and bcl-2 was detected by SABC method. MTT assay and flow cytometry.Results Antisense PCNA with the concentration of 6.5 ~ 100μmol · L-1 had a inhibitory rate of 8.3% ~ 38.4% on RPE in a dose-dependent manner and inhibited the growth of cells PCNA expression antisense bcl-2 showed no inhibitory effect.Conclusion Antisense PCNA deoxyol oligonucleotide can inhibit the growth of nearly 40% of RPE, its clinical application deserves further study