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目的 探讨甲状腺激素对骨发育调节作用的分子机制。本文从体内和体外实验两方面研究了甲状腺激素对BMP 2、IGF 1基因的调节作用。方法 复制碘缺乏大鼠动物模型 ,检测了甲状腺激素缺乏状态下大鼠颅骨中BMP 2、IGF 1基因表达水平。原代培养正常和碘缺乏大鼠颅骨成骨细胞 ,加入T3 观察成骨细胞中BMP 2、IGF 1因表达水平的变化。结果 BMP 2、IGF 1基因表达水平在碘缺乏大鼠颅骨中明显降低。 1、14、2 8、4 2、5 6、84各日龄正常组大鼠颅骨中BMP 2mRNA表达量分别为 3.2、4 .0、0 .75、0 .6 2、0 .4 6和 0 .31× 10 -16mol·μg-1总RNA ,碘缺乏组分为 0 .9、0 .5 2、0 .2 0、0 .16、0 .13和 0 .2 0× 10 -16mol·μg-1总RNA ,分别为正常组的 1/ 6 .5、1/ 7.6 9、1/ 3.75、1/ 3.88、1/ 3.5 4和 1/ 1.5 5。各日龄正常大鼠IGF 1相对表达量 (IGF 1与 β actin的灰度值比 )为 1.2 8± 0 .17、1.2 7± 0 .18、1.14± 0 .16、0 .84± 0 .0 7、0 .4 8± 0 .10和 0 .89± 0 .0 8;碘缺乏大鼠分别为 1.0 4± 0 .0 4、1.19± 0 .12、1.16± 0 .0 2、0 .4 5± 0 .0 5、0 .4 0± 0 .0 3和 0 .6 5± 0 .0 5 ,分别较正常组降低了 2 3%、6 .7%、7.5 %、87%、2 0 %和 37%。体外实验中 ,T3 浓度从 0 ,10 -10 ～ 10 -6mol·L-1?
Objective To explore the molecular mechanism of thyroid hormone regulating bone development. In this study, we investigated the regulatory effect of thyroid hormones on BMP 2 and IGF 1 genes in vitro and in vivo. Methods The iodine deficiency rat model was duplicated and the expression of BMP 2 and IGF 1 gene in rat skull under thyroid hormone deficiency was detected. Primary cultures of normal and iodine-deficient rat calvarial osteoblasts were performed. The expression of BMP 2 and IGF-1 in osteoblasts was detected by adding T3. Results The BMP 2 and IGF 1 gene expression levels were significantly decreased in the iodine-deficient rat skull. 1,14,28,4 2,5 6,84 BMP 2 mRNA expression in the normal rat skull at each age were 3.2,4 .0,0 .75,0 .6 2,0 .4 6 and 0 .31 × 10 -16mol · μg-1 total RNA, iodine-deficient components were 0.9,0.5 2,0 .2,0,0 .16,0 .13 and0.2 × 10 -16mol · μg -1 total RNA were 1/6 .5, 1 / 7.6 9, 1 / 3.75, 1 / 3.88, 1 / 3.5 4 and 1 / 1.5 5 respectively in the normal group. The relative expression level of IGF 1 (the ratio of the gray value of IGF 1 to β actin) in normal rats of each day was 1.2 8 ± 0.17, 1.2 7 ± 0.18, 1.14 ± 0.16, 0.84 ± 0. 0 7,0 .4 8 ± 0 .10 and 0 .89 ± 0. 0 8; iodine deficient rats were 1.0 4 ± 0 .0 4,1.19 ± 0.12,1.16 ± 0 .0 2,0 respectively. 4 5 ± 0 .0 5,0 .4 0 ± 0 .0 3 and 0 .6 5 ± 0 .0 5, respectively, compared with the normal group decreased 23%, 6.7%, 7.5%, 87%, 2 0% and 37%. In vitro experiments, T3 concentration from 0, 10 -10 ~ 10 -6mol · L-1?