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目的检测尿路致病性大肠杆菌 (uropathogenicEscherichiacoli,UPEC)临床株I型菌毛的携带频率 ,并对其粘附素基因FimH作序列分析。方法用血凝方法检测尿路致病性大肠杆菌的I型菌毛 ;根据GENEBANK的大肠杆菌I型菌毛粘附素FimH基因序列设计引物 ,用聚合酶链反应 (polymerasechainreaction ,PCR)检测临床分离的 15 5株UPEC的目的基因 ,选取典型阳性株的扩增产物测序。结果 15 5株尿路致病性大肠杆菌 ,用血凝方法仅检测到 5 5株携有I型菌毛 ,阳性率 3 5 .5 % ;而用基因扩增方法检测 14 0株 (90 .5 % )含有FimH基因。扩增产物序列与尿路致病性大肠杆菌标准株J96的序列基本一致。结论FimH基因存在于大多数尿路致病性大肠杆菌临床株中 ,是其重要毒力因子 ,具有重要的临床意义。
Objective To detect the frequency of type I pili in uropathogenic Escherichia coli (UPEC) clinical strains and analyze the sequence of the adhesin gene FimH. Methods The type I fimbriae of pathogenic Escherichia coli in urinary tract were detected by hemagglutination method. Primers were designed according to the sequence of FimH gene of Escherichia coli type I pili of GENEBANK. The clinical isolates were detected by polymerase chain reaction (PCR) Of 15 5 strains of UPEC gene, select the typical positive strains amplified product sequencing. Results Fifty-five strains of pathogenic Escherichia coli in urinary tract were detected. Only 5 5 strains of pilus were detected by hemagglutination method, with a positive rate of 35.5%. 14 0 strains were detected by gene amplification method. 5%) contains the FimH gene. The sequence of amplified product was consistent with the sequence of urinary tract pathogenic E. coli strain J96. Conclusion FimH gene exists in most of the pathogenic E. coli clinical strains of urinary tract and is an important virulence factor, which has important clinical significance.