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目的 探讨As2 O3 经肝动脉栓塞化对肝脏的毒副作用及临床意义。方法 采用生理盐水 (实验组 1)、生理盐水 +碘化油 (实验组 2 )、As2 O3 +碘化油 (实验组 3 )经肝动脉注射治疗兔肝移植瘤一次 ,As2 O3 (实验组 4)经肝动脉插管灌注药物 ,连续 7d。观察瘤旁肝细胞、肝细胞线粒体的形态学变化并行线粒体比表面积定量分析、肝功能检测。结果 实验组 2、3肝细胞不同程度细胞体积增大、浊肿、甚至气球样变 ,实验组 4除肝细胞不同程度细胞体积增大、浊肿、气球样变外 ,还可见灶性坏死。实验组 3与实验组 4相比差异有显著性 (P <0 0 0 0 1) ,与实验组 2比差异无显著性 (P >0 0 5 )。肝细胞线粒体比表面积定量分析显示实验组 1、2、3、4肝细胞的线粒体比表面积逐渐缩小 ,差异具有显著性 ( P <0 0 5 ) ,同一实验组肝细胞线粒体比表面积与肿瘤细胞线粒体比表面积相比 ,同样具有差异性 (P <0 0 5 )。实验组 2、3、4肝功能指标中LDH、AST、ALT不同程度升高 ,实验组 2、3分别与实验组 1、4比较差异有显著性 (P <0 0 5 ) ;实验组 3与实验组 2比差异无显著性 (P >0 0 5 )。结论 As2 O3 经肝动脉栓塞化疗对兔肝细胞有一定的损伤作用 ,但程度轻于As2 O3 经肝动脉灌注治疗对肝脏的毒副作用。预示经肝动脉栓塞化疗是
Objective To investigate the toxic side effects and clinical significance of As2O3 on hepatic artery embolization by hepatic artery. Methods Transplanted hepatic tumor xenografts were treated with normal saline (experimental group 1), saline + iodized oil (experimental group 2), As 2 O 3 + iodized oil (experimental group 3) ) Intragastric catheterization of drugs, continuous 7d. Morphological changes of mitochondria of para-tumorous hepatocytes and hepatocytes were observed, and quantitative analysis of specific surface area of mitochondria and liver function were performed. Results In experimental group 2 and 3, the number of hepatocytes increased, the turbidity was swollen, and even the balloon-like changes. In experimental group 4, in addition to varying degrees of hepatocytes, cell volume increased, turbidity and balloon-like changes, focal necrosis was also seen. The difference between experimental group 3 and experimental group 4 was significant (P <0 0 001), but there was no significant difference between experimental group 3 and experimental group 2 (P 0 05). Quantitative analysis of mitochondrial specific surface area of liver cells showed that the mitochondrial specific surface area of hepatocytes in experimental groups 1, 2, 3 and 4 gradually decreased, and the difference was significant (P <0.05). The mitochondrial specific surface area of hepatocytes in the same experimental group was significantly lower than that of mitochondria Compared with the surface area, also has the difference (P <0 0 5). The LDH, AST and ALT levels of experimental group 2, 3 and 4 increased to different extents. There was significant difference between experimental group 2,3 and experimental group 1 and 4 (P <0.05) There was no significant difference in experimental group 2 (P> 0.05). Conclusion As 2 O 3 transcatheter hepatic arterial chemoembolization has a certain damage effect on rabbit hepatocytes, but to a lesser degree than As 2 O 3 transhepatic arterial perfusion treatment of liver toxicity. Predictors of hepatic arterial chemoembolization are