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目的:观察高山红景天对大鼠实验性肝纤维化的治疗效果,并探讨其作用机制。方法:用四氯化碳诱导大鼠肝纤维化模型,将实验动物随机分为正常对照组(A)、模型组(B)、秋水仙碱组(C)、红景天高剂量组(D)、红景天低剂量组(E)。除正常对照组外,其余4组均用四氯化碳诱发肝纤维化,并相应灌药8周。各组于造模第8周末处死动物,分别用放射免疫法检测血清层黏连蛋白(LN),Ⅲ型前胶原(PCⅢ),透明质酸(HA)及Ⅳ型胶原(CⅣ);RT-PCR检测肝组织金属蛋白酶组织抑制因子-1(TIMP-1)mRNA的表达;肝组织HE染色和胶原纤维染色。结果:与模型组大鼠比较,各用药组大鼠血清中LN、HA、PCⅢ、CⅣ水平明显降低(P<0.01);大鼠肝组织内TIMP-1mRNA表达明显下降(P<0.01);大鼠肝组织病理学检测改善显著。结论:高山红景天能有效地抑制肝纤维化的发展,其机制可能是通过下调肝组织内TIMP-1mRNA表达,降低ECM的分泌而达到的。
Objective: To observe the therapeutic effect of Rhodiola sachalinensis on experimental hepatic fibrosis in rats and its mechanism of action. Methods: The rat model of hepatic fibrosis was induced by carbon tetrachloride. The experimental animals were randomly divided into normal control group (A), model group (B), colchicine group (C), rhodiola high dose group ), Rhodiola low dose group (E). In addition to the normal control group, the remaining four groups were induced by carbon tetrachloride liver fibrosis, and the corresponding perfusion for 8 weeks. Animals in each group were sacrificed at the end of the 8th week. Serum levels of laminin (LN), PCⅢ, HA and C Ⅳ were detected by radioimmunoassay. RT- PCR was used to detect the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA in liver tissue; HE staining and collagen fiber staining in liver tissue. Results: Compared with the model group, the levels of LN, HA, PCⅢ and CⅣ in the serum of rats in each treatment group were significantly decreased (P <0.01); the expression of TIMP-1 mRNA in the liver tissue was significantly decreased Histopathological examination of rat liver improved significantly. Conclusion: Rhodiola sachalinensis can effectively inhibit the development of hepatic fibrosis, and its mechanism may be achieved by down-regulating the expression of TIMP-1 mRNA in liver tissue and decreasing the secretion of ECM.